Heterologous expression of secreted biologically active human interleukin-10 in Bifidobacterium breve

Archives of Microbiology
E V KhokhlovaA N Shkoporov

Abstract

Construction of Bifidobacterium breve capable of production of secreted biologically active human interleukin-10 (hIL-10) is described. ORF coding for full-length mature human interleukin-10 was cloned into a series of expression vectors. This resulted in generation of translational fusions between hIL-10 and signal peptides sequences derived from Bifidobacterium breve genes sec2, apuB and B. adolescentis gene amyB under the control of constitutively active bifidobacterial promoter. We have shown that fusion to amyB signal peptide resulted in highest expression level of hIL-10 at the mRNA and protein level. Secreted hIL-10 was highly unstable in bifidobacterial culture supernatants in standard growth conditions. However, incubation of stationary cultures in buffered tissue culture medium resulted in production of stable biologically active hIL-10, albeit in low amounts (1.9 ng/ml).

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Citations

May 15, 2012·Applied and Environmental Microbiology·Zhongke SunChristian U Riedel
Dec 5, 2014·Microbial Cell Factories·Olivia Cano-GarridoElena García-Fruitós
May 23, 2015·FEMS Microbiology Letters·A N ShkoporovB A Efimov
Dec 29, 2012·Beneficial Microbes·S GuglielmettiP Álvarez-Martín
Mar 4, 2014·Applied and Environmental Microbiology·Verena GrimmChristian U Riedel
Jan 10, 2019·Frontiers in Microbiology·Aurélie MaurasAnne-Judith Waligora-Dupriet
Jan 21, 2021·Microbial Cell Factories·Nayoun HongMyeong Soo Park

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