Heterologous expression of the acyl-acyl carrier protein thioesterase gene from the plant Umbellularia californica mediates polyhydroxyalkanoate biosynthesis in recombinant Escherichia coli

Applied Microbiology and Biotechnology
B H Rehm, A Steinbüchel

Abstract

The acyl-acyl carrier protein (ACP) thioesterase cDNA from the plant Umbellularia californica was functionally expressed in various recombinant Escherichia coli strains in order to establish a new metabolic route toward medium-chain-length polyhydroxyalkanoate (PHA(MCL)) biosynthesis from non-related carbon sources. Coexpression of the PHA synthase genes from Ralstonia eutropha and Pseudomonas aeruginosa, or only the PHA synthase gene from P. aeruginosa, respectively, showed PHA(MCL) accumulation when the type II PHA synthase from P. aeruginosa was produced. Both wild-type E. coli and various fad mutants were investigated; and only when the beta-oxidation pathway was impaired PHA(MCL) accumulation from gluconate was observed, contributing to about 6% of cellular dry weight. Thus coexpression of type II PHA synthase gene with cDNA encoding the medium-chain acyl-ACP thioesterase from U. californica established a new PHA(MCL) biosynthesis pathway, connecting fatty acid de novo biosynthesis with fatty acid beta-oxidation, using a non-related carbon source.

Citations

Jul 5, 2007·Applied Microbiology and Biotechnology·Qian LiuGuo-Qiang Chen
Mar 12, 2013·Applied Microbiology and Biotechnology·Qian WangQingsheng Qi
Jan 11, 2014·Biotechnology for Biofuels·Helge Jans Janßen, Alexander Steinbüchel
Nov 13, 2012·Metabolic Engineering·Daniel E AgnewBrian F Pfleger
Dec 7, 2011·Journal of Bioscience and Bioengineering·Alexander P Mueller, Christopher T Nomura
Nov 14, 2006·Bioresource Technology·Rui LiQingsheng Qi
Apr 2, 2013·Current Opinion in Biotechnology·Rebecca M Lennen, Brian F Pfleger
Mar 10, 2016·Journal of Biotechnology·Michael WierzbickiCong T Trinh
Jun 7, 2015·Applied Microbiology and Biotechnology·David O Hooks, Bernd H A Rehm

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