Heterologous expression, purification, crystallization and preliminary X-ray analysis of raucaffricine glucosidase, a plant enzyme specifically involved in Rauvolfia alkaloid biosynthesis

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Martin RuppertJoachim Stöckigt

Abstract

Raucaffricine glucosidase (RG) is an enzyme that is specifically involved in the biosynthesis of indole alkaloids from the plant Rauvolfia serpentina. After heterologous expression in Escherichia coli cells, crystals of RG were obtained by the hanging-drop vapour-diffusion technique at 293 K with 0.3 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 buffer and 11% PEG 4000 as precipitant. Crystals belong to space group I222 and diffract to 2.30 A, with unit-cell parameters a = 102.8, b = 127.3, c = 215.8 A.

References

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Apr 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sylvestre MarillonnetYuri Gleba
Sep 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chotima PoeaknapoMeinhart H Zenk
Jan 25, 2005·The Journal of Biological Chemistry·Xueyan MaJoachim Stöckigt
Jun 3, 2005·Acta Crystallographica. Section D, Biological Crystallography·Juergen KoepkeJoachim Stöckigt
Jun 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Chotima BoettcherMeinhart H Zenk

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Citations

Dec 5, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Cindy RosenthalJoachim Stöckigt
Feb 7, 2008·Annual Review of Plant Biology·Jörg Ziegler, Peter J Facchini
Jun 19, 2012·Phytochemistry·Liqun XiaJoachim Stoeckigt
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Peter J Facchini, Vincenzo De Luca
Aug 24, 2010·Natural Product Reports·Liuqing Yang, Joachim Stöckigt
Oct 19, 2011·ACS Chemical Biology·Liqun XiaJoachim Stöckigt

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