Heterologous microarray experiments allow the identification of the early events associated with potato tuber cold sweetening.

BMC Genomics
Paolo BagnaresiPierdomenico Perata

Abstract

Since its discovery more than 100 years ago, potato (Solanum tuberosum) tuber cold-induced sweetening (CIS) has been extensively investigated. Several carbohydrate-associated genes would seem to be involved in the process. However, many uncertainties still exist, as the relative contribution of each gene to the process is often unclear, possibly as the consequence of the heterogeneity of experimental systems. Some enzymes associated with CIS, such as beta-amylases and invertases, have still to be identified at a sequence level. In addition, little is known about the early events that trigger CIS and on the involvement/association with CIS of genes different from carbohydrate-associated genes. Many of these uncertainties could be resolved by profiling experiments, but no GeneChip is available for the potato, and the production of the potato cDNA spotted array (TIGR) has recently been discontinued. In order to obtain an overall picture of early transcriptional events associated with CIS, we investigated whether the commercially-available tomato Affymetrix GeneChip could be used to identify which potato cold-responsive gene family members should be further studied in detail by Real-Time (RT)-PCR (qPCR). A tomato-potato Global Matc...Continue Reading

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Citations

Aug 14, 2013·BMC Genomics·Paolo BagnaresiEmidio Sabatini
Dec 15, 2010·BMC Plant Biology·Astrid M DraffehnChristiane Gebhardt
Dec 22, 2012·BMC Plant Biology·João Paulo FabiJoão Roberto Oliveira do Nascimento
Nov 28, 2015·Molecular Breeding : New Strategies in Plant Improvement·Dorota Sołtys-KalinaWaldemar Marczewski
Feb 27, 2017·Functional & Integrative Genomics·Jonathan NeilsonH H Tai
Jan 1, 2014·Microarrays·Yick Ching WongHarikrishna Kulaveerasingam
Nov 26, 2019·Plant Physiology and Biochemistry : PPB·Michele Valquíria Dos ReisSchuyler S Korban

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Methods Mentioned

BETA
chip
transgenic
environmental stress
electrophoresis

Software Mentioned

TIGR
ALIGN
R
BLAST
GeneChip
TIGR TA
GCRMA
Affymetrix
Magellan
PERF

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