Heterologous Processing and Export of the Bacteriocins Pediocin PA-1 and Lactococcin A in Lactococcus Lactis: A Study with Leader Exchange

Probiotics and Antimicrobial Proteins
M L ChikindasK Venema

Abstract

The bacteriocins pediocin PA-1 and lactococcin A are synthesized as precursors carrying N-terminal extensions with a conserved cleavage site preceded by two glycine residues in positions -2 and -1. Each bacteriocin is translocated through the cytoplasmic membrane by an integral membrane protein of the ABC cassette superfamily which, in the case of pediocin PA-1, has been shown to possess peptidase activity responsible for proteolytic cleavage of the pre-bacteriocin. In each case, another integral membrane protein is essential for bacteriocin production. In this study, a two-step PCR approach was used to permutate the leaders of pediocin PA-1 and lactococcin A. Wild-type and chimeric pre-bacteriocins were assayed for maturation by the processing/export machinery of pediocin PA-1 and lactococcin A. The results show that pediocin PA-1 can be efficiently exported by the lactococcin machinery whether it carries the lactococcin or the pediocin leader. It can also compete with wild-type lactococcin A for the lactococcin machinery. Pediocin PA-1 carrying the lactococcin A leader or lactococcin A carrying that of pediocin PA-1 was poorly secreted when complemented with the pediocin PA-1 machinery, showing that the pediocin machinery is ...Continue Reading

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Citations

Jul 20, 2006·Microbial Cell Factories·Catherine M BurgessDouwe van Sinderen
Sep 8, 2005·Microbial Cell Factories·Jacob Glenting, Stephen Wessels
Jul 29, 2008·Research in Microbiology·Séverine LayecNathalie Leblond-Bourget
Jul 1, 2008·International Journal of Food Microbiology·Etienne DakoRonald E Simard
Jun 24, 2004·FEMS Microbiology Letters·Charlotte BeltramoJean Guzzo
Nov 6, 2004·International Journal of Food Microbiology·Yolanda SanzBert Poolman
Aug 24, 2004·FEMS Microbiology Letters·M Cruz MartínMiguel A Alvarez
Jun 13, 2012·The Journal of General and Applied Microbiology·Esteban Vera PingitoreMar Iacutea Elena Nader-Macías
Apr 10, 2019·Polymers·Tianqi HuangChuncai Zhou
Nov 19, 2019·Journal, Genetic Engineering & Biotechnology·Tejinder KaurBaljinder Kaur
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Małgorzata Gumienna, Barbara Górna

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