Abstract
Hyaluronic acid (HA) is used in a wide range of medical applications, where its performance and therapeutic efficacy are highly dependent on its molecular weight. In the microbial production of HA, it has been suggested that a high level of intracellular ATP enhances the productivity and molecular weight of HA. Here, we report on heterologous HA production in an ε-poly-l-lysine producer, Streptomyces albulus, which has the potential to generate ATP at high level. The hasA gene from Streptococcus zooepidemicus, which encodes HA synthase, was refactored and expressed under the control of a late-log growth phase-operating promoter. The expression of the refactored hasA gene, along with genes coding for UDP-glucose dehydrogenase, UDP-N-acetylglucosamine pyrophosphorylase, and UDP-glucose pyrophosphorylase, which are involved in HA precursor sugar biosynthesis, resulted in efficient production of HA in the 2.0 MDa range, which is greater than typical bacterial HA, demonstrating that a sufficient amount of ATP was provided to support the biosynthesis of the precursor sugars, which in turn promoted HA production. In addition, unlike in the case of streptococcal HA, S. albulus-derived HA was not cell associated. Based on these findings...Continue Reading
References
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M R WesselsT J DiCesare
Dec 1, 1972·Analytical Biochemistry·R F Itzhaki
Dec 1, 1994·International Journal of Biological Macromolecules·M O'ReganM Lansing
Oct 12, 2000·The Journal of Biological Chemistry·C HeldermonP H Weigel
Mar 7, 2001·Journal of Clinical Microbiology·K M TownsendB Adler
Jul 28, 2001·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·N Adam, P Ghosh
Feb 1, 1956·Journal of General Microbiology·A P MACLENNAN
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Dec 16, 2004·Applied Microbiology and Biotechnology·Barrie Fong ChongLars K Nielsen
Jul 8, 2005·Applied and Environmental Microbiology·Bill WidnerSteve Brown
Oct 20, 2005·Journal of Bioscience and Bioengineering·Yoshimitsu HamanoHiroshi Takagi
Jul 1, 1997·Applied and Environmental Microbiology·D C Armstrong, M R Johns
Jan 24, 2007·The Journal of Biological Chemistry·Hitoshi TakahashiMasanori Sugiyama
Feb 3, 2007·The Journal of Bone and Joint Surgery. American Volume·Daniel J Sucato
Jun 14, 2008·Journal of Molecular Evolution·Lars M BlankLars K Nielsen
Nov 11, 2008·Nature Chemical Biology·Kazuya YamanakaYoshimitsu Hamano
Mar 25, 2009·Applied Microbiology and Biotechnology·Zichao MaoRachel Chen
May 20, 2009·The Journal of Biological Chemistry·Wendy Yiting ChenLars Keld Nielsen
Jul 6, 2010·Applied and Environmental Microbiology·Kazuya YamanakaYoshimitsu Hamano
Mar 11, 2011·Journal of Bioscience and Bioengineering·Kazuya YamanakaYoshimitsu Hamano
Nov 18, 2011·Microbial Cell Factories·Long LiuJian Chen
Jan 5, 2013·Journal of Bioscience and Bioengineering·Naoko KitoYoshimitsu Hamano
Sep 7, 2013·Genome Announcements·Amanda DoddKarl Rumbold
Apr 20, 2014·Genome Announcements·Zhaoxian XuZhuzhen Sun
Citations
Jun 24, 2016·Applied Microbiology and Biotechnology·Zhaoxian XuHong Xu
Jul 3, 2016·Microbial Cell Factories·Juliana Davies de OliveiraNádia Skorupa Parachin
Dec 31, 2015·Current Opinion in Biotechnology·Jian WangYajun Yan
Feb 1, 2018·Biotechnology and Bioengineering·Adam W WestbrookC Perry Chou
Sep 25, 2017·Biotechnology and Bioengineering·Adam W WestbrookC Perry Chou
Mar 28, 2017·Applied Microbiology and Biotechnology·Zhengshan LuoJian Chen
Aug 31, 2019·Microorganisms·Antonio M V GomesNádia S Parachin
Mar 31, 2020·Biotechnology Advances·Yueyang FengYingjin Yuan
Feb 8, 2020·Trends in Biotechnology·Xianhao XuLong Liu
Aug 14, 2020·International Journal of Biological Macromolecules·Vijay GunasekaranPonnusami V
Jul 24, 2021·Carbohydrate Polymers·Yibin QiuErzheng Su
Aug 10, 2021·Frontiers in Bioengineering and Biotechnology·Mario A Torres-AcostaCuauhtemoc Licona-Cassani
Jan 15, 2019·Journal of Agricultural and Food Chemistry·Delei XuHong Xu
Jul 31, 2021·Journal of Applied Microbiology·João H C Manfrão-NettoNádia Skorupa Parachin
Sep 11, 2021·Scientific Reports·Arezoo NazeriFarzaneh Aram