Apr 2, 2005

Heterologous protein production from the inducible MET25 promoter in Saccharomyces cerevisiae

Biotechnology Progress
Steven P SolowMichael W Laird

Abstract

Heterologous protein production late in Saccharomyces cerevisiae fermentations is often desirable because it may help avoid the unintentional selection of more rapidly growing, non-protein-expressing cells or allow for the expression of toxic proteins. Here, we describe the use of the MET25 promoter for the production of human serum albumin (HSA) and HSA-fusion proteins in S. cerevisiae. In media lacking methionine, the MET25 promoter yielded high expression levels of HSA and HSA fused to human glucagon, human growth hormone, human interferon alpha, and human interleukin-2. More importantly, we have shown that this system can be used to delay heterologous protein production until late log phase of the growth of the culture and does not require the addition of an exogenous inducer.

Mentioned in this Paper

Saccharomyces cerevisiae Proteins
Glucagon (rDNA)
Chimeric Proteins, Recombinant
Saccharomyces cerevisiae allergenic extract
SDS-PAGE
Albumin Human, USP
Promoter
Human Serum Albumin [EPC]
Glucagon
Pedameth

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