Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis

BioMed Research International
Sun Hee KimJong Bum Kim

Abstract

Reconstitution of nonnative, very-long-chain polyunsaturated fatty acid (VLC-PUFA) biosynthetic pathways in Arabidopsis thaliana was undertaken. The introduction of three primary biosynthetic activities to cells requires the stable coexpression of multiple proteins within the same cell. Herein, we report that C22 VLC-PUFAs were synthesized from C18 precursors by reactions catalyzed by Δ(6)-desaturase, an ELOVL5-like enzyme involved in VLC-PUFA elongation, and Δ(5)-desaturase. Coexpression of the corresponding genes (McD6DES, AsELOVL5, and PtD5DES) under the control of the seed-specific vicilin promoter resulted in production of docosapentaenoic acid (22:5 n-3) and docosatetraenoic acid (22:4 n-6) as well as eicosapentaenoic acid (20:5 n-3) and arachidonic acid (20:4 n-6) in Arabidopsis seeds. The contributions of the transgenic enzymes and endogenous fatty acid metabolism were determined. Specifically, the reasonable synthesis of omega-3 stearidonic acid (18:4 n-3) could be a useful tool to obtain a sustainable system for the production of omega-3 fatty acids in seeds of a transgenic T3 line 63-1. The results indicated that coexpression of the three proteins was stable. Therefore, this study suggests that metabolic engineering ...Continue Reading

References

Mar 8, 1974·Molecular and Cellular Biochemistry·R R Brenner
May 1, 1993·The American Journal of Clinical Nutrition·D F Horrobin
Jul 21, 1998·The Journal of Biological Chemistry·L V MichaelsonA K Stobart
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Jul 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J M Parker-BarnesP Mukerji
Jun 6, 2003·Bioscience, Biotechnology, and Biochemistry·Eiji Sakuradani, Sakayu Shimizu
Jan 21, 2004·Phytochemistry·Olga V Sayanova, Johnathan A Napier
Mar 8, 2005·Trends in Plant Science·Frédéric DomergueErnst Heinz
Jul 29, 2005·The Proceedings of the Nutrition Society·Johnathan A Napier, Olga Sayanova
Sep 27, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Morris K AgabaAlan J Teale
Feb 13, 2007·Current Opinion in Biotechnology·Ian A GrahamJohnathan A Napier
Oct 22, 2008·BMC Research Notes·Luis Oñate-Sánchez, Jesús Vicente-Carbajosa
Nov 13, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Melissa K GregoryKathryn A Schuller
Jun 10, 2010·Fish Physiology and Biochemistry·N Y Mohd-YusofD R Tocher
Jul 19, 2011·Progress in Lipid Research·Hemant PoudyalLindsay Brown
Jul 1, 2005·Functional Plant Biology : FPB·Stan S RobertAllan G Green

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Citations

Jul 9, 2020·Progress in Lipid Research·Sandra M CorreaYariv Brotman

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Datasets Mentioned

BETA
GQ352540

Methods Mentioned

BETA
transgenic
PCR
transgenics

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