Heteromeric KV 2/KV 8.2 Channels Mediate Delayed Rectifier Potassium Currents in Primate Photoreceptors

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Jacqueline Gayet-PrimoTeresa Puthussery

Abstract

Silent voltage-gated potassium channel subunits (KVS) interact selectively with members of the KV2 channel family to modify their functional properties. The localization and functional roles of these silent subunits remain poorly understood. Mutations in the KVS subunit, KV8.2 (KCNV2), lead to severe visual impairment in humans, but the basis of these deficits remains unclear. Here, we examined the localization, native interactions, and functional properties of KV8.2-containing channels in mouse, macaque, and human photoreceptors of either sex. In human retina, KV8.2 colocalized with KV2.1 and KV2.2 in cone inner segments and with KV2.1 in rod inner segments. KV2.1 and KV2.2 could be coimmunoprecipitated with KV8.2 in retinal lysates indicating that these subunits likely interact directly. Retinal KV2.1 was less phosphorylated than cortical KV2.1, a difference expected to alter the biophysical properties of these channels. Using voltage-clamp recordings and pharmacology, we provide functional evidence for Kv2-containing channels in primate rods and cones. We propose that the presence of KV8.2, and low levels of KV2.1 phosphorylation shift the activation range of KV2 channels to align with the operating range of rod and cone pho...Continue Reading

Citations

Apr 2, 2019·PLoS Biology·James C R GroveSteven Barnes
Jan 28, 2020·The Journal of General Physiology·Norianne T IngramGordon L Fain
Mar 20, 2020·Investigative Ophthalmology & Visual Science·Moritz LindnerMark W Hankins
May 23, 2020·Ophthalmic Genetics·Thales A C De GuimaraesMichel Michaelides
Sep 25, 2020·International Journal of Molecular Sciences·Meha Kabra, Bikash Ranjan Pattnaik
Mar 17, 2019·Documenta Ophthalmologica. Advances in Ophthalmology·Tomoko KutsumaTadashi Nakano
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Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Samantha C O'DwyerL Fernando Santana
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Sep 29, 2021·The Journal of General Physiology·Parashar ThapaJon T Sack
Oct 16, 2021·Human Molecular Genetics·Shivangi M InamdarSheila A Baker

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