PMID: 8357793Aug 24, 1993

Heteronuclear nuclear magnetic resonance studies of cobalt corrinoids. 15. The structure of glutathionylcobalamin: a 1H and 13C two-dimensional nuclear magnetic resonance study at 600 MHz

Biochemistry
K L BrownD W Jacobsen

Abstract

Glutathionylcobalamin (GSCbl), the complex formed between glutathione (GSH, gamma-glutamylcysteinylglycine) and aquacobalamin (H2OCbl), has been implicated as an intermediate in the pathway for the formation of the cobalamin coenzymes. In chemical model studies, GSCbl has been shown to be a substrate for methylcobalamin formation in the presence of S-adenosylmethionine and a thiol reductant. Although GSCbl was first described in 1964, the structure of this compound, particularly the site of GSH coordination, has been unknown. GSCbl was prepared by reacting GSH (5-fold molar excess) with H2OCbl in 0.1 M sodium phosphate (pH 6.5) and was purified by gel-permeation chromatography on a Bio-Gel P2 polyacrylamide column. By use of a combination of homonuclear [homonuclear J-correlated spectroscopy (COSY), homonuclear Hartmann-Hahn spectroscopy (HOHAHA), and absorption-mode nuclear Overhauser effect spectroscopy (NOESY)] and inverse detected heteronuclear [1H-detected heteronuclear multiple-quantum coherence (HMQC) and 1H-detected multiple-bond heteronuclear multiple-quantum coherence (HMBC) spectroscopies] two-dimensional NMR methods at 600 MHz, the complete 1H and 13C NMR spectra of GSCbl have now been assigned. Comparison of the 1H...Continue Reading

References

Jun 15, 1990·Biochemical and Biophysical Research Communications·E PezackaD W Jacobsen
Nov 6, 1984·Biochemistry·G T Bratt, H P Hogenkamp
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

Citations

Jun 22, 1999·Journal of Inorganic Biochemistry·Nicola E Brasch, R G Finke
Sep 25, 2010·Inorganic Chemistry·Luciana HannibalD W Jacobsen
May 10, 2012·The Journal of Physical Chemistry. a·Azaria S EisenbergRonald L Birke
Mar 7, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Keishiro TaharaYoshio Hisaeda
Mar 28, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Christopher B PerryHelder M Marques
Feb 26, 2008·Chembiochem : a European Journal of Chemical Biology·Peter SommerTamis Darbre
Jun 19, 2012·Journal of Porphyrins and Phthalocyanines·Somasundaram RamasamyJoseph M Rifkind

Related Concepts

Glutathionylcobalamin
Carbon Isotopes
Cobalt
Reduced Glutathione
Hydrogen
In Vivo NMR Spectroscopy
Eritron

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