Heterophilic antibody interference in a non-endogenous molecule assay: an apparent elevation in the tacrolimus concentration

Clinica Chimica Acta; International Journal of Clinical Chemistry
Sara AltinierMario Plebani

Abstract

In drug monitoring assays the most common interferences are due to hematocrit, other drugs or their metabolites, while the interference by heterophilic antibodies has been reported only when measuring endogenous molecules. In the present paper a heterophilic antibody interference in the tacrolimus measurement is described. Samples from a patient treated with tacrolimus were analyzed on RxL Dimension analyzer. Ranging drug concentrations from 49 to 12.5 microg/L, even after the interruption of the treatment, confirmation analysis were performed using heterophilic blocking tubes before tacrolimus measurement on the same analyzer, then testing the samples on V-Twin System, finally incubating the samples with chlorophenol red beta-d-galactopyranoside, beta-galactosidase, polyclonal mouse IgG, protein A and Protein G resin. The elevated tacrolimus concentrations were due to the presence of an interference attributable to heterophilic antibodies, as confirmed by treating the samples with heterophilic blocking tubes and protein G resin. a) The interference caused by heterophilic antibodies can be found not only in immunoassays measuring endogenous molecules, but also in those for exogenous molecules; b) the pre-treatment sample proced...Continue Reading

References

Sep 23, 2003·Clinical Chemistry·Paul N SpanC G J Sweep
Jul 13, 2004·Clinical Pharmacokinetics·Christine E Staatz, Susan E Tett
Jun 26, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Megan A GriffeyMitchell G Scott
Oct 30, 2007·Drug Metabolism and Pharmacokinetics·Kazuhide Iwasaki

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Citations

Feb 22, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Johann MorelleEric Goffin
Nov 12, 2013·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Kazuaki TaguchiHideyuki Saito
Dec 9, 2014·Bioanalysis·Ricardo Jorge Dinis-Oliveira
Nov 10, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mariadomenica D'AlessandroAlessandra Bachetoni
Aug 3, 2010·Journal of Pharmaceutical and Biomedical Analysis·Christophe BazinAnne Hulin
Oct 20, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Donatella MoscatoAntonio Caropreso
Mar 25, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·M ZaninottoM Plebani
Apr 9, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Massimo TempestilliLeopoldo P Pucillo
Mar 11, 2010·Therapeutic Drug Monitoring·Bijal A ParikhMitchell G Scott
Jul 14, 2010·Therapeutic Drug Monitoring·Hylke de JongeDirk R Kuypers
Aug 2, 2013·Clinical Chemistry·Thomas M AnnesleyDonald S Mason
May 1, 2019·Combinatorial Chemistry & High Throughput Screening·Ataman Gönel, Ismail Koyuncu

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