Heterotrimeric Galphaq11 co-immunoprecipitates with surface-anchored GRP78 from plasma membranes of alpha2M*-stimulated macrophages

Journal of Cellular Biochemistry
Uma K Misra, Salvatore V Pizzo

Abstract

We have previously shown that a fraction of newly expressed GRP78 is translocated to the cell surface in association with the co-chaperone MTJ-1. Proteinase and methylamine-activated alpha(2)M (alpha(2)M*) bind to cell surface-associated GRP78 activating phosphoinositide-specific phospholipase C coupled to a pertussis toxin-insensitive heterotrimeric G protein, generating IP(3)/calcium signaling. We have now studied the association of pertussis toxin-insensitive Galphaq11, with GRP78/MTJ-1 complexes in the plasma membranes of alpha(2)M*-stimulated macrophages. When GRP78 was immunoprecipitated from plasma membranes of macrophages stimulated with alpha(2)M*, Galphaq11, and MTJ-1 were co-precipitated. Likewise Galphaq11 and GRP78 co-immunoprecipitated with MTJ-1 while GRP78 and MTJ-1 co-immunoprecipitated with Galphaq11. Silencing GRP78 expression with GRP78 dsRNA or MTJ-1 with MTJ-1 dsRNA greatly reduced the levels of Galphaq11 co-precipitated with GRP78 or MTJ-1. In conclusion, we show here that plasma membrane-associated GRP78 is coupled to pertussis toxin-insensitive Galphaq11 and forms a ternary signaling complex with MTJ-1.

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Citations

Apr 25, 2012·Histochemistry and Cell Biology·Sriram RavindranAnne George
Jan 22, 2010·Apoptosis : an International Journal on Programmed Cell Death·U K Misra, S V Pizzo
Oct 20, 2009·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·John A PapalasMaria A Selim
Apr 1, 2009·Antioxidants & Redox Signaling·Mario Gonzalez-GronowSalvatore V Pizzo
Sep 25, 2009·Journal of Virology·Tomoyuki HondaKeizo Tomonaga
Mar 23, 2011·Virology Journal·Yi-Ping WuRobert Yung-Liang Wang
Aug 31, 2020·Journal of Cellular Physiology·Udhayakumar Gopal, Salvatore V Pizzo

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