Heterotrimeric NADH-oxidizing methylenetetrahydrofolate reductase from the acetogenic bacterium Acetobacterium woodii

Journal of Bacteriology
Johannes BertschVolker Müller

Abstract

The methylenetetrahydrofolate reductase (MTHFR) of acetogenic bacteria catalyzes the reduction of methylene-THF, which is highly exergonic with NADH as the reductant. Therefore, the enzyme was suggested to be involved in energy conservation by reducing ferredoxin via electron bifurcation, followed by Na(+) translocation by the Rnf complex. The enzyme was purified from Acetobacterium woodii and shown to have an unprecedented subunit composition containing the three subunits RnfC2, MetF, and MetV. The stable complex contained 2 flavin mononucleotides (FMN), 23.5 ± 1.2 Fe and 24.5 ± 1.5 S, which fits well to the predicted six [4Fe4S] clusters in MetV and RnfC2. The enzyme catalyzed NADH:methylviologen and NADH:ferricyanide oxidoreductase activity but also methylene-tetrahydrofolate (THF) reduction with NADH as the reductant. The NADH:methylene-THF reductase activity was high (248 U/mg) and not stimulated by ferredoxin. Furthermore, reduction of ferredoxin, alone or in the presence of methylene-THF and NADH, was never observed. MetF or MetVF was not able to catalyze the methylene-THF-dependent oxidation of NADH, but MetVF could reduce methylene-THF using methyl viologen as the electron donor. The purified MTHFR complex did not cata...Continue Reading

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Citations

Jan 21, 2016·Biotechnology for Biofuels·Okkyoung Choi, Byoung-In Sang
Dec 23, 2015·Biotechnology for Biofuels·Johannes Bertsch, Volker Müller
Sep 15, 2016·Extremophiles : Life Under Extreme Conditions·Mirko Basen, Volker Müller
Oct 14, 2016·Frontiers in Microbiology·Jongoh ShinByung-Kwan Cho
Feb 23, 2018·Environmental Microbiology·Viola KrukenbergGunter Wegener
Jun 21, 2018·Annual Review of Microbiology·Volker MüllerMirko Basen
Jun 21, 2015·Applied and Environmental Microbiology·Johannes Bertsch, Volker Müller
Aug 24, 2018·Environmental Microbiology·Mats LechtenfeldVolker Müller
Sep 23, 2018·Applied and Environmental Microbiology·Judith Dönig, Volker Müller
Jul 14, 2018·Environmental Microbiology·Florian KrempVolker Müller
Aug 21, 2018·Frontiers in Microbiology·Saroj PoudelEric S Boyd
Jan 31, 2021·Microorganisms·Anja Wiechmann, Volker Müller
Jan 12, 2021·Frontiers in Bioengineering and Biotechnology·Alexander Katsyv, Volker Müller
Sep 10, 2020·FEMS Microbiology Reviews·Florian Kremp, Volker Müller
Jun 15, 2021·Environmental Microbiology·Helge M DietrichVolker Müller
Jul 14, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Christian ÖppingerVolker Müller
Sep 5, 2021·Extremophiles : Life Under Extreme Conditions·Florian P Rosenbaum, Volker Müller
Oct 15, 2021·Extremophiles : Life Under Extreme Conditions·Alexander KatsyvVolker Müller

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