Heterozygous embryonic lethality induced by targeted inactivation of the VEGF gene

Nature
N FerraraM W Moore

Abstract

Angiogenesis is required for a wide variety of physiological and pathological processes. The endothelial cell-specific mitogen vascular endothelial growth factor (VEGF) is a major mediator of pathological angiogenesis. Also, the expression of VEGF and its two receptors, Flt-1 and Flk-1/KDR, is related to the formation of blood vessels in mouse and rat embryos. Mice homozygous for mutations that inactivate either receptor die in utero between days 8.5 and 9.5. However, ligand(s) other than VEGF might activate such receptors. To assess the role of VEGF directly, we disrupted the VEGF gene in embryonic stem cells. Here we report the unexpected finding that loss of a single VEGF allele is lethal in the mouse embryo between days 11 and 12. Angiogenesis and blood-island formation were impaired, resulting in several developmental anomalies. Furthermore, VEGF-null embryonic stem cells exhibit a dramatically reduced ability to form tumours in nude mice.

References

May 1, 1992·Molecular and Cellular Biology·R M MortensenJ G Seidman
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D MaglioneM G Persico
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J D'AmatoJ Folkman
Jun 1, 1995·Current Biology : CB·E P BrandonG S McKnight
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K G PetersL T Williams
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T P QuinnL T Williams

❮ Previous
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Citations

Sep 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·O CleaverP A Krieg
Mar 31, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·X ZengJ A Whitsett
May 29, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M AitkenheadJ Wilting
May 26, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K AaseU Eriksson
May 26, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·B M Weinstein
Sep 4, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·X Yue, R J Tomanek
Apr 15, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J Lough, Y Sugi
Nov 20, 1997·Journal of Cellular Physiology·I FlammeW Risau
Nov 20, 1997·Journal of Cellular Physiology·V JoukovK Alitalo
May 18, 2000·Journal of Morphology·J Eriksson, J Löfberg
Jan 1, 1997·Developmental Genetics·J L RinkenbergerZ Werb
Oct 22, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L Robb, A G Elefanty
Aug 30, 2000·Cancer·F YamasakiK Kurisu
Nov 7, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A M HealyW V Cardoso
Nov 21, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·B D Abbott, A R Buckalew
Nov 21, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K IshiguroT Muramatsu
Jan 9, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T J PooleC M Cox
Feb 13, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y S NgP A D'Amore
Feb 13, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y M LeeK W Kim
Nov 4, 2000·International Journal of Cancer. Journal International Du Cancer·H DingA Guha
Apr 25, 2001·Journal of Biomedical Materials Research·D J LymanE S Wijelath
Jun 27, 2000·Journal of Cellular Physiology·F MontaldoM Prat
Jul 27, 2000·Journal of Cellular Physiology·S F HackettP A Campochiaro
Jul 27, 2000·Journal of Cellular Physiology·P A Campochiaro
Dec 13, 2000·Diabetes/metabolism Research and Reviews·S ChakrabartiS Chen
Sep 14, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B L Roman, B M Weinstein
Jun 17, 1998·Arthritis and Rheumatism·A E Koch
Jul 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·James M GreenbergAnn L Akeson
Nov 2, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·W Scott ArgravesChristopher J Drake
Jan 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·I AjiokaY Watanabe
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·J LuoM Korc
Oct 13, 2001·Journal of Magnetic Resonance Imaging : JMRI·S P WilliamsN van Bruggen
Oct 23, 1997·Protein Science : a Publication of the Protein Society·W J FairbrotherM A Starovasnik
Mar 15, 1997·Developmental Biology·A Tufro-McReddieR A Gomez
Dec 17, 1997·Developmental Biology·K R KozakO Hankinson
Dec 4, 1998·Springer Seminars in Immunopathology·N Oppenheimer-Marks, P E Lipsky
Feb 11, 2005·Folia Microbiologica·J Rajnoch, O Viklický
Dec 15, 2005·Archives of Pharmacal Research·Sung-Jin Lee, Hak-Kyo Lee
Nov 28, 2001·International Journal of Hematology·T Suda, N Takakura
May 21, 2005·Cellular and Molecular Life Sciences : CMLS·A A TimurQ Wang
Mar 12, 2013·Cellular and Molecular Life Sciences : CMLS·Ina M Wittko-SchneiderKarl H Plate
Sep 25, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kevin WeiCalvin J Kuo
May 6, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ryan C RiddleThomas L Clemens
Mar 3, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jincai LuoYao Lu

❮ Previous
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