Heterozygous embryonic stem cell lines derived from nonhuman primate parthenotes

Stem Cells
Vikas DigheShoukhrat Mitalipov

Abstract

Monoparental parthenotes represent a potential source of histocompatible stem cells that should be isogenic with the oocyte donor and therefore suitable for use in cell or tissue replacement therapy. We generated five rhesus monkey parthenogenetic embryonic stem cell (PESC) lines with stable, diploid female karyotypes that were morphologically indistinguishable from biparental controls, expressed key pluripotent markers, and generated cell derivatives representative of all three germ layers following in vivo and in vitro differentiation. Interestingly, high levels of heterozygosity were observed at the majority of loci that were polymorphic in the oocyte donors. Some PESC lines were also heterozygous in the major histocompatibility complex region, carrying haplotypes identical to those of the egg donor females. Expression analysis revealed transcripts from some imprinted genes that are normally expressed from only the paternal allele. These results indicate that limitations accompanying the potential use of PESC-derived phenotypes in regenerative medicine, including aberrant genomic imprinting and high levels of homozygosity, are cell line-dependent and not always present. PESC lines were derived in high enough yields to be pra...Continue Reading

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Citations

Oct 13, 2010·Cellular Reprogramming·Maryam PashaiaslPaul J Verma
Jun 5, 2010·Human Reproduction·Hathaitip SritanaudomchaiShoukhrat Mitalipov
Nov 6, 2009·Genome Research·Netta Mendelson CohenAmos Tanay
Sep 10, 2013·Stem Cells and Development·Masumi HirabayashiShinichi Hochi
Jun 13, 2008·Stem Cell Reviews·Tiziana A L BreviniFulvio Gandolfi
Jul 4, 2015·Annals of Medicine·Oscar E SimonsonSergey Rodin
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Jun 3, 2009·Stem Cells·Michelle SparmanShoukhrat Mitalipov
Mar 19, 2016·Development, Growth & Differentiation·Guang-Yu BaiLei Lei
Dec 17, 2009·Aging Cell·Junjiu HuangDavid L Keefe
Nov 26, 2015·Biochimica Et Biophysica Acta·Kaja BreckwoldtThomas Eschenhagen
Dec 5, 2015·Stem Cells International·Adriana Bos-MikichFlávio V Meirelles
Jan 21, 2014·Stem Cells Translational Medicine·Brittany Daughtry, Shoukhrat Mitalipov
Mar 1, 2008·Regenerative Medicine·Don P Wolf
Jul 7, 2018·Circulation Research·Jianyi ZhangGordana Vunjak-Novakovic

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