Heuristically optimal path scanning for high-speed multiphoton circuit imaging

Journal of Neurophysiology
Alexander J SadovskyJason N MacLean

Abstract

Population dynamics of patterned neuronal firing are fundamental to information processing in the brain. Multiphoton microscopy in combination with calcium indicator dyes allows circuit dynamics to be imaged with single-neuron resolution. However, the temporal resolution of fluorescent measures is constrained by the imaging frequency imposed by standard raster scanning techniques. As a result, traditional raster scans limit the ability to detect the relative timing of action potentials in the imaged neuronal population. To maximize the speed of fluorescence measures from large populations of neurons using a standard multiphoton laser scanning microscope (MPLSM) setup, we have developed heuristically optimal path scanning (HOPS). HOPS optimizes the laser travel path length, and thus the temporal resolution of neuronal fluorescent measures, using standard galvanometer scan mirrors. Minimizing the scan path alone is insufficient for prolonged high-speed imaging of neuronal populations. Path stability and the signal-to-noise ratio become increasingly important factors as scan rates increase. HOPS addresses this by characterizing the scan mirror galvanometers to achieve prolonged path stability. In addition, the neuronal dwell time ...Continue Reading

References

Apr 30, 2005·Nature Reviews. Neuroscience·Kenneth D Harris
Dec 13, 2005·Neuron·Jason N MacLeanRafael Yuste
Dec 5, 2006·Nature Methods·Werner GöbelFritjof Helmchen
Oct 30, 2007·Nature Methods·Volodymyr NikolenkoRafael Yuste
Apr 25, 2008·Nature Neuroscience·Gaddum Duemani ReddyPeter Saggau
Jun 10, 2008·Journal of Neuroscience Methods·Kyle P LillisJerome Mertz
Dec 19, 2008·PloS One·Brendon O WatsonRafael Yuste
Jan 9, 2009·Frontiers in Neural Circuits·Volodymyr NikolenkoRafael Yuste
Jun 18, 2010·Journal of Neurophysiology·Joshua T VogelsteinLiam Paninski
Jul 9, 2010·Journal of Neurophysiology·Ilya ValmianskiDavid Kleinfeld
Jan 11, 2011·Nature Methods·Adrian ChengCarlos Portera-Cailliau

Citations

Jul 13, 2012·PLoS Computational Biology·Eftychios A PnevmatikakisLiam Paninski
Aug 30, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexander J Sadovsky, Jason N MacLean
Aug 20, 2016·PLoS Computational Biology·Brendan Chambers, Jason N MacLean
May 6, 2016·Journal of Neurophysiology·Charles G Frye, Jason N MacLean
Nov 14, 2017·Journal of Neural Engineering·Renaud SchuckSimon R Schultz
Oct 11, 2013·Nature Communications·Peter B KruskalJason N MacLean
Aug 30, 2016·Nature Neuroscience·Na JiSpencer L Smith
Aug 30, 2016·Nature Neuroscience·Kenneth D HarrisSpencer L Smith
Jul 19, 2013·Journal of Neural Engineering·Jon OñativiaPier Luigi Dragotti
Jan 31, 2020·PLoS Computational Biology·Subhodh Kotekal, Jason N MacLean
May 30, 2014·Journal of Neurophysiology·Melissa J RunfeldtJason N MacLean
Jul 21, 2017·Journal of Neurophysiology·Joseph B Dechery, Jason N MacLean
Feb 20, 2015·Journal of Neurophysiology·Audrey J SederbergJason N MacLean
Jul 24, 2015·Journal of Neurophysiology·Brendan Chambers, Jason N MacLean
Jun 19, 2018·Network Neuroscience·Brendan ChambersJason N MacLean

Related Concepts

Nerve Impulses
Mice, Inbred C57BL
Neurons
Computer Programs and Programming
Microscopy, Fluorescence, Multiphoton
Brain
Diagnostic Imaging
Dyes
Enzyme Stability
Fluorescent Dyes

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.