Hexadecameric structure of an invertebrate gap junction channel

Journal of Molecular Biology
Atsunori OshimaYoshinori Fujiyoshi

Abstract

Innexins are invertebrate-specific gap junction proteins with four transmembrane helices. These proteins oligomerize to constitute intercellular channels that allow for the passage of small signaling molecules associated with neural and muscular electrical activity. In contrast to the large number of structural and functional studies of connexin gap junction channels, few structural studies of recombinant innexin channels are reported. Here we show the three-dimensional structure of two-dimensionally crystallized Caenorhabditis elegans innexin-6 (INX-6) gap junction channels. The N-terminal deleted INX-6 proteins are crystallized in lipid bilayers. The three-dimensional reconstruction determined by cryo-electron crystallography reveals that a single INX-6 gap junction channel comprises 16 subunits, a hexadecamer, in contrast to chordate connexin channels, which comprise 12 subunits. The channel pore diameters at the cytoplasmic entrance and extracellular gap region are larger than those of connexin26. Two bulb densities are observed in each hemichannel, one in the pore and the other at the cytoplasmic side of the hemichannel in the channel pore pathway. These findings imply a structural diversity of gap junction channels among ...Continue Reading

References

Jan 21, 1977·Science·I SimpsonW R Loewenstein
Aug 1, 1977·The Journal of Cell Biology·L MakowskiD A Goodenough
Jan 1, 1992·Electron Microscopy Reviews·B Leitch
Jun 1, 1991·Journal of Structural Biology·S S SikerwarR M Glaeser
Feb 16, 1984·Nature·P N Unwin, P D Ennis
Mar 24, 1994·Nature·V K VerselisT A Bargiello
Jan 1, 1996·Journal of Structural Biology·R A CrowtherJ M Smith
Feb 1, 1997·Biophysical Journal·G PerkinsG Sosinsky
Oct 14, 1998·Trends in Genetics : TIG·P PhelanR J Wyman
Jul 19, 2000·Current Biology : CB·Y PanchinS Lukyanov
Apr 22, 2004·Journal of Structural Biology·Nobuhiko GyobuYoshinori Fujiyoshi
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Aug 12, 2004·FEBS Letters·Li BaoGerhard Dahl
Jun 1, 2005·Biochimica Et Biophysica Acta·Pauline Phelan
Jun 28, 2006·IUBMB Life·Gerhard Dahl, Silviu Locovei
Jun 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Atsunori OshimaGina E Sosinsky
Aug 24, 2007·The Journal of Biological Chemistry·Daniela BoassaGina Sosinsky
Nov 24, 2007·FEBS Letters·Li BaoGerhard Dahl
Jul 19, 2008·Neuron Glia Biology·Eliana ScemesDavid C Spray
Jul 24, 2008·Cell Communication & Adhesion·Atsunori OshimaGina E Sosinsky
Jul 31, 2008·Journal of Cell Science·John W KyleEric C Beyer
Oct 15, 2008·Pflügers Archiv : European journal of physiology·Eliana ScemesPaolo Meda
Apr 3, 2009·Nature·Shoji MaedaTomitake Tsukihara
May 30, 2009·The Journal of Biological Chemistry·John W KyleEric C Beyer
Nov 26, 2010·Journal of Molecular Biology·Atsunori OshimaYoshinori Fujiyoshi
Apr 26, 2011·Journal of Structural Biology·Yasumi OhtaYoshinori Fujiyoshi
May 3, 2011·Channels·Gina E SosinskyGerhard Dahl
Oct 8, 2011·Biochimica Et Biophysica Acta·Thaddeus A BargielloTaekyung Kwon
Mar 1, 2012·Biochimica Et Biophysica Acta·Federico Abascal, Rafael Zardoya
Mar 6, 2013·The Journal of Biological Chemistry·Atsunori OshimaYoshinori Fujiyoshi
Jan 2, 2014·Journal of Neurophysiology·William D Marks, I Martha Skerrett
Feb 5, 2014·FEBS Letters·Atsunori Oshima
Feb 28, 2014·Frontiers in Physiology·Karina T SimonsenChristian C Naus
Aug 19, 2015·Structure·Florian HauerHolger Stark

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Citations

Sep 21, 2016·Current Opinion in Neurobiology·Lidia Szczupak
Feb 2, 2017·Proceedings of the National Academy of Sciences of the United States of America·Heeun JangCornelia I Bargmann
Jun 1, 2017·Biochimica Et Biophysica Acta. Biomembranes·Eric C Beyer, Viviana M Berthoud
Dec 3, 2016·Nature Communications·Atsunori OshimaYoshinori Fujiyoshi
Sep 2, 2016·Developmental Neurobiology·I Martha Skerrett, Jamal B Williams
Aug 12, 2016·Developmental Neurobiology·Michael W Baker, Eduardo R Macagno
Jun 14, 2016·Developmental Neurobiology·David H Hall
Feb 26, 2020·Science Advances·Batuujin BurendeiAtsunori Oshima
Nov 26, 2020·Developmental Cell·Lingfeng Meng, Dong Yan
Apr 19, 2021·Journal of Molecular Biology·Johanna SyrjanenHiro Furukawa
Nov 11, 2021·Open Biology·Jorge Henrique NevesGlaucia Maria Machado-Santelli

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