PMID: 9555051May 23, 1998Paper

Hexadecylphosphocholine and 2-modified 1,3-diacylglycerols as effectors of phospholipase D

Biochimica Et Biophysica Acta
N DittrichRenate Ulbrich-Hofmann

Abstract

The kinetic behaviour of phospholipase D (PLD) from cabbage has been studied in the presence of several substrate-like compounds such as hexadecylphosphocholine (HPC) and 1,3-didodecanoylglycero-2-phosphatides. 1,3-Didodecanoyl- glycero-2-phosphocholine (1,3-DiC12PC) was found being not cleft by PLD, whereas HPC is hydrolyzed by PLD with small rate. The plot of initial velocity vs. substrate concentration for HPC is more sigmoidal than those for the common substrate phosphatidylcholine (PC)/sodium dodecylsulfate (SDS) (1:0.5) or the short-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DiC6PC). The anionic amphiphiles 1,3-didodecanoylglycero-2-sulfate and 1,3-didodecanoylglycero-2-phosphate act as activators of PLD towards PC similar to SDS. In contrast, 1,3-DiC12PC shows inhibitory properties with an increase in the sigmoidicity of the initial velocity as a function of substrate concentration in the PC/SDS assay. Also HPC inhibits the hydrolysis of PC/SDS, whereas it acts as activator or inhibitor in the hydrolysis of DiC6PC. The results suggest that PLD possesses two substrate-binding sites, where one binds substrate in function of an effector without catalytic activity while the other is the catalytic site. HPC and 1,3-DiC...Continue Reading

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Citations

Nov 6, 2002·Journal of Experimental Therapeutics & Oncology·Dinko BerkovicEduard A M Fleer
Sep 17, 2002·European Journal of Biochemistry·José M Jiménez-LópezCarmen Marco
Jan 5, 2011·Chemistry and Physics of Lipids·Johanna MansfeldRenate Ulbrich-Hofmann
Mar 17, 2009·Biochimica Et Biophysica Acta·Johanna Mansfeld, Renate Ulbrich-Hofmann
Jun 10, 2015·Phytochemistry·Hafeeza KhatoonRenate Ulbrich-Hofmann
Mar 14, 2008·Chembiochem : a European Journal of Chemical Biology·Atsushi MasayamaYugo Iwasaki
Oct 24, 2008·Chembiochem : a European Journal of Chemical Biology·Konstantin KuppeRenate Ulbrich-Hofmann
Jun 24, 2005·Biotechnology Letters·Renate Ulbrich-HofmannLydia Bezakova

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