Hexane Extract of Orthosiphon stamineus Induces Insulin Expression and Prevents Glucotoxicity in INS-1 Cells

Diabetes & Metabolism Journal
Hae-Jung LeeYong Woon Kim

Abstract

Hyperglycemia, a characteristic feature of diabetes, induces glucotoxicity in pancreatic β-cells, resulting in further impairment of insulin secretion and worsening glycemic control. Thus, preservation of insulin secretory capacity is essential for the management of type 2 diabetes. In this study, we evaluated the ability of an Orthosiphon stamineus (OS) extract to prevent glucotoxicity in insulin-producing cells. We measured insulin mRNA expression and glucose-stimulated insulin secretion (GSIS) in OS-treated INS-1 cells after exposure to a high glucose (HG; 30 mM) concentration. The hexane extract of OS elevated mRNA expression of insulin as well as pancreatic and duodenal homeobox-1 of INS-1 cells in a dose-dependent manner. The hexane OS extract also increased the levels of phosphorylated phosphatidylinositol 3-kinase (PI3K) in a concentration-dependent manner. Additionally, Akt phosphorylation was elevated by treatment with 100 and 200 µmol of the hexane OS extract. Three days of HG exposure suppressed insulin mRNA expression and GSIS; these expressions were restored by treatment with the hexane OS extract. HG elevated peroxide levels in the INS-1 cells. These levels were unaffected by OS treatment under both normal and hy...Continue Reading

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Citations

Jun 22, 2017·Drug and Chemical Toxicology·Dhamraa Waleed Al-DualimiAmin Malik Shah Abdul Majid
Jan 16, 2015·The Korean Journal of Internal Medicine·Jae-Seung Yun, Seung-Hyun Ko
May 22, 2019·International Journal of Molecular Sciences·Krista Minéia WartchowPatrícia Sesterheim

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Methods Mentioned

BETA
enzyme-linked
ELISA
flow cytometry

Software Mentioned

EXPO
Multi Gauge

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