Abstract
Fowl adenovirus (FAdV) is distributed worldwide and causes economic losses in the poultry industry. The objectives of this study were to determine the hexon and fiber gene changes in an attenuated FAdV isolate from Malaysia in specific pathogen-free chicken embryonated eggs (SPF CEE) and its infectivity in commercial broiler chickens. SPF CEE were inoculated with 0.1 mL FAdV inoculum via the chorioallantoic membrane (CAM) for 20 consecutive passages. The isolate at passage 20 (E20), with a virus titer of 108.7TCID50/mL (TCID50, 50% tissue culture infective dose), was inoculated (0.5 mL) into one-day-old commercial broiler chicks either via oral or intraperitoneal routes. The study demonstrated that 100% embryonic mortality was recorded from E2 to E20 with a delayed pattern at E17 onwards. The lesions were confined to the liver and CAM. Substitutions of amino acids in the L1 loop of hexon at positions 49 and 66, and in the knob of fiber at positions 318 and 322 were recorded in the E20 isolate. The isolate belongs to serotype 8b and is non-pathogenic to broiler chickens, but it is able to induce a FAdV antibody titer. It appears that molecular changes in the L1 loop of hexon and the knob of fiber are markers for FAdV infectivity.
References
Aug 1, 1996·Journal of Virology·J PallisterM Sheppard
Oct 10, 1998·Journal of Virological Methods·R Raue, M Hess
Apr 28, 2007·Avian Diseases·I R AlvaradoL B Purvis
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Jan 1, 1977·Avian Pathology : Journal of the W.V.P.A·C ItakuraM Goto
Dec 1, 2001·Avian Pathology : Journal of the W.V.P.A·G MeulemansM Decaesstecker
Sep 22, 2010·The Journal of Infectious Diseases·Hongbo ZhouMeilin Jin
Sep 30, 2010·Tropical Animal Health and Production·Muhammad Khalid MansoorGhulam Muhammad
Jan 18, 2011·Virus Research·Helena GrgićEva Nagy
Apr 15, 2011·Poultry Science·K Domanska-BlicharzZ Minta
Apr 19, 2011·Australian Veterinary Journal·P A SteerA H Noormohammadi
May 7, 2011·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 28, 2011·Journal of Comparative Pathology·W AlemneshA R Omar
Oct 22, 2013·Veterinary Microbiology·Győző L KajánMária Benkő
Oct 22, 2013·Virus Genes·Helena GrgićEva Nagy
Jan 1, 2015·Journal of the South African Veterinary Association·Louis H MaartensEstelle H Venter
Jul 1, 2017·Avian Diseases·Rima MorshedSaeid Charkhkar
Citations
Dec 24, 2019·Avian Diseases·Michael Haryadi WibowoGusti Ngurah Mahardika
Jan 1, 2020·PloS One·Norfitriah Mohamed SohaimiNurulfiza Mat Isa
Jun 15, 2019·Viruses·Guanliu YuYouxiang Diao
Nov 3, 2020·Veterinary World·Otto Sahat Martua SilaenChristian Marco Hadi Nugroho
Mar 13, 2021·Frontiers in Veterinary Science·Zhipeng JiaDexing Ma
Jul 28, 2021·Journal of Veterinary Science·Nur Syazana SabarudinAbdul Rahman Omar
Aug 3, 2021·Avian Diseases·Rahul KumarSunil K Mor