PMID: 6386465Oct 15, 1984Paper

Hexose metabolism in pancreatic islets. The phosphorylation of fructose

European Journal of Biochemistry
A SenerW J Malaisse

Abstract

Fructose, like glucose, rapidly equilibrates across the plasma membrane of pancreatic islet cells, but is poorly metabolized and is a weak insulin secretagogue in rat pancreatic islets. A possible explanation for such a situation was sought by investigating the modality of fructose phosphorylation in islet homogenates. Several findings indicated that the phosphorylation of fructose is catalyzed by hexokinase, but not fructokinase. First, at variance with the situation found in liver homogenates, the phosphorylation of fructose in the islet homogenate was unaffected by K+ and inhibited by glucose, mannose, glucose 6-phosphate or glucose 1,6-bisphosphate. Second, the Km for fructose was much higher in islets than in liver. Third, in islet homogenates the Km and Vmax for fructose were much higher than those for glucose or mannose phosphorylation, at low aldohexose concentrations, in good agreement with the properties of purified hexokinase. In intact islets fructose augmented the islet content in glucose 6-phosphate sufficiently to cause marked inhibition of its own rate of phosphorylation. These findings may account, in part at least, for the low rate of fructose utilization by rat pancreatic islets.

References

Aug 15, 1978·The Biochemical Journal·S J AshcroftC J Hedeskov
Feb 1, 1973·The Biochemical Journal·S J AshcroftP J Randle
Apr 1, 1973·Diabetologia·P M Dean
Oct 1, 1973·Diabetologia·L A Idahl
Jun 1, 1970·The Biochemical Journal·S J AshcroftP J Randle
Jun 1, 1971·Canadian Journal of Physiology and Pharmacology·B J Lin, R E Haist
Jul 6, 1971·Biochimica Et Biophysica Acta·B HellmanI B Täljedal
Dec 1, 1972·Endocrinology·D L CurryM Gomez
Jan 13, 1971·Biochimica Et Biophysica Acta·J J SánchezH G Pontis
Mar 15, 1984·Biochemical and Biophysical Research Communications·M H GiroixW J Malaisse
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D MeglassonF M Matschinsky
Oct 15, 1984·The Biochemical Journal·M H GiroixW J Malaisse
Mar 15, 1983·The Biochemical Journal·W J MalaisseJ Christophe
Feb 11, 1982·Biochemical and Biophysical Research Communications·A SenerW J Malaisse
Jul 30, 1982·Biochemical and Biophysical Research Communications·M Van de WinkleD G Pipeleers

Citations

Mar 1, 1987·General and Comparative Endocrinology·P Ronner, A Scarpa
Jan 1, 1987·The International Journal of Biochemistry·F Malaisse-Lagae, W J Malaisse
Jan 1, 1991·The International Journal of Biochemistry·W J Malaisse, H Bodur
Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·M H GiroixW J Malaisse
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M S German
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·H HeimbergF Schuit
Dec 5, 2006·The Journal of Biological Chemistry·Nathan L VanderfordSabire Ozcan
Dec 30, 1987·European Journal of Biochemistry·A SenerW J Malaisse
Jul 5, 1990·European Journal of Biochemistry·W J MalaisseE Van Schaftingen
Jan 1, 1986·Diabetes/metabolism Reviews·M D Meglasson, F M Matschinsky
Sep 11, 1989·FEBS Letters·W J MalaisseE Van Schaftingen
Apr 1, 1996·Scandinavian Journal of Clinical and Laboratory Investigation·D MalmJ Florholmen
Oct 16, 2020·Current Diabetes Reports·Robert N HelsleySamir Softic

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