Hfq plays important roles in virulence and stress adaptation in Cronobacter sakazakii ATCC 29544

Infection and Immunity
Seongok KimSangryeol Ryu

Abstract

Cronobacter spp. are opportunistic pathogens that cause neonatal meningitis and sepsis with high mortality in neonates. Despite the peril associated with Cronobacter infection, the mechanisms of pathogenesis are still being unraveled. Hfq, which is known as an RNA chaperone, participates in the interaction with bacterial small RNAs (sRNAs) to regulate posttranscriptionally the expression of various genes. Recent studies have demonstrated that Hfq contributes to the pathogenesis of numerous species of bacteria, and its roles are varied between bacterial species. Here, we tried to elucidate the role of Hfq in C. sakazakii virulence. In the absence of hfq, C. sakazakii was highly attenuated in dissemination in vivo, showed defects in invasion (3-fold) into animal cells and survival (10(3)-fold) within host cells, and exhibited low resistance to hydrogen peroxide (10(2)-fold). Remarkably, the loss of hfq led to hypermotility on soft agar, which is contrary to what has been observed in other pathogenic bacteria. The hyperflagellated bacteria were likely to be attributable to the increased transcription of genes associated with flagellar biosynthesis in a strain lacking hfq. Together, these data strongly suggest that hfq plays import...Continue Reading

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Citations

Dec 20, 2015·Future Microbiology·Joana R FelicianoJorge H Leitão
Jan 18, 2018·Scientific Reports·Seongok KimSangryeol Ryu
Jun 6, 2017·Frontiers in Microbiology·Rute G MatosCecília M Arraiano
Nov 23, 2017·Frontiers in Microbiology·Han-Yueh KuoMing-Li Liou
Oct 24, 2020·Pathogens and Disease·Josicelli Souza CrispimDenise Mara Soares Bazzolli
Nov 7, 2020·Microbial Pathogenesis·Esteban-Kenel VeronicaCruz-Córdova Ariadnna

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