Hfq stimulates the activity of the CCA-adding enzyme

BMC Molecular Biology
Marion ScheibeMario Mörl


The bacterial Sm-like protein Hfq is known as an important regulator involved in many reactions of RNA metabolism. A prominent function of Hfq is the stimulation of RNA polyadenylation catalyzed by E. coli poly(A) polymerase I (PAP). As a member of the nucleotidyltransferase superfamily, this enzyme shares a high sequence similarity with an other representative of this family, the tRNA nucleotidyltransferase that synthesizes the 3'-terminal sequence C-C-A to all tRNAs (CCA-adding enzyme). Therefore, it was assumed that Hfq might not only influence the poly(A) polymerase in its specific activity, but also other, similar enzymes like the CCA-adding enzyme. Based on the close evolutionary relation of these two nucleotidyltransferases, it was tested whether Hfq is a specific modulator acting exclusively on PAP or whether it also influences the activity of the CCA-adding enzyme. The obtained data indicate that the reaction catalyzed by this enzyme is substantially accelerated in the presence of Hfq. Furthermore, Hfq binds specifically to tRNA transcripts, which seems to be the prerequisite for the observed effect on CCA-addition. The increase of the CCA-addition in the presence of Hfq suggests that this protein acts as a stimulating...Continue Reading


Jul 11, 1989·Biochemistry·K B HallA G Redfield
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R Sampson, Olke C Uhlenbeck
Aug 16, 1974·Nature·J D RobertusA Klug
Jan 31, 1998·The Journal of Biological Chemistry·N B ReuvenM P Deutscher
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·Eliane Hajnsdorf, Philippe Régnier
Aug 18, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T A AzamA Ishihama
Mar 17, 2001·Molecular Microbiology·Anna BalandinaJosette Rouviere-Yaniv
Oct 11, 2001·Biological Chemistry·H SchürerM Mörl
Jan 24, 2002·Molecular Cell·Thorleif MøllerPoul Valentin-Hansen
May 15, 2002·The Journal of Biological Chemistry·Anna BalandinaJosette Rouviere-Yaniv
Jul 11, 2003·Nucleic Acids Research·Claude SauterDietrich Suck
Nov 19, 2003·Molecular Microbiology·Aixia ZhangSusan Gottesman
Dec 5, 2003·Nucleic Acids Research·Marc FolichonEliane Hajnsdorf
Jan 24, 2004·The EMBO Journal·Thomas A Geissmann, Danièle Touati
Mar 11, 2004·Molecular Microbiology·Poul Valentin-HansenChristina Udesen
Sep 24, 2004·Biochemical and Biophysical Research Communications·Elisabeth SonnleitnerUdo Bläsi
Nov 4, 2004·Molecular Microbiology·Bijoy K MohantySidney R Kushner
Nov 9, 2004·Nature Structural & Molecular Biology·Peter J MikuleckyAndrew L Feig
Nov 25, 2004·Journal of Molecular Biology·Richard A Lease, Sarah A Woodson
Oct 6, 2005·Genes & Development·Klas I UdekwuE Gerhart H Wagner
Oct 18, 2005·Nature Structural & Molecular Biology·Amy H BuckNorman R Pace
Nov 30, 2005·Molecular Microbiology·Anders Aamann RasmussenPoul Valentin-Hansen
Dec 13, 2005·Journal of Molecular Biology·Véronique ArluisonSergio Marco
Feb 2, 2006·Nucleic Acids Research·Katarzyna ZiolkowskaEliane Hajnsdorf


Feb 20, 2010·BMC Molecular Biology·Jacques Le DeroutEliane Hajnsdorf
Feb 16, 2010·Cellular and Molecular Life Sciences : CMLS·Heike BetatMario Mörl
Jan 16, 2016·Current Opinion in Microbiology·Nicholas R De Lay, Danielle A Garsin
Oct 18, 2011·Journal of Molecular Biology·Takao IgarashiYa-Ming Hou
Dec 4, 2015·Journal of Basic Microbiology·Lei YangHua Tang
Feb 9, 2017·Nature Communications·Dennis KappeiFalk Butter
Jul 16, 2011·Nature Reviews. Microbiology·Jörg Vogel, Ben F Luisi
Jul 29, 2020·International Journal of Molecular Sciences·Lieselotte ErberM Mörl
Dec 3, 2020·International Journal of Molecular Sciences·Oliver HennigM Mörl
Jan 21, 2021·Nucleic Acids Research·Anna EnderM Mörl

Methods Mentioned


Related Concepts

CCA-adding enzyme, human
PcnB protein, E coli
Hfq protein, E coli
Enzyme Activation
Polynucleotide Adenylyltransferase
Plasma Protein Binding Capacity
RNA Nucleotidyltransferases
Transfer RNA
Substrate Specificity
Escherichia coli Proteins

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