HGF and BFGF Secretion by Human Adipose-Derived Stem Cells Improves Ovarian Function During Natural Aging via Activation of the SIRT1/FOXO1 Signaling Pathway

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Chenyue DingBoxian Huang

Abstract

Human adipose-derived stem cells (hADSCs) are a potential therapeutic option for clinical applications because of their ability to produce cytokines and their capacity for trilineage differentiation. To date, few researchers have investigated the effects of hADSCs on natural ovarian aging (NOA). An NOA mouse model and human ovarian granule cells (hGCs) collected from individuals with NOA were prepared to assess the therapeutic effects and illuminate the mechanism of hADSCs in curing NOA. Enzyme-linked immunosorbent assay was used to detect the serum levels of sex hormones and antioxidative enzymes. The proliferation rate and marker expression level of hGCs were measured by flow cytometry (FACS). Cytokines were measured by a protein antibody array methodology. Western blot assays were used to determine the protein expression levels of SIRT1 and FOXO1. Our results showed that hADSCs displayed therapeutic activity against ovarian function in an NOA mouse model, increasing the proliferation rate and marker expression level of hGCs. Furthermore, the yields of hADSC-secreted HGF and bFGF were higher than those of other growth factors. FACS showed that combination treatment with the growth factors HGF and bFGF more strongly promoted p...Continue Reading

Citations

Jun 5, 2020·Stem Cells Translational Medicine·Rhian Stavely, Kulmira Nurgali
Mar 14, 2019·International Journal of Molecular Medicine·Guiping LuoYingbin Xiao
Dec 5, 2020·Journal of Ethnopharmacology·Tingting LouXiangyan Li
Apr 10, 2021·Reproductive Sciences·P PetricM Stimpfel
Oct 15, 2021·Journal of Ovarian Research·Xing-Hua PanGuang-Ping Ruan
Nov 4, 2021·The Journal of Obstetrics and Gynaecology Research·Qin WangShiyan Sui

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