Hh and Wnt signaling regulate formation of olig2+ neurons in the zebrafish cerebellum.

Developmental Biology
Karen A McFarlandBruce Appel

Abstract

The cerebellum, which forms from anterior hindbrain, coordinates motor movements and balance. Sensory input from the periphery is relayed and modulated by cerebellar interneurons, which are organized in layers. The mechanisms that specify the different neurons of the cerebellum and direct its layered organization remain poorly understood. Drawing from investigations of spinal cord, we hypothesized that the embryonic cerebellum is patterned on the dorsoventral axis by opposing morphogens. We tested this using zebrafish. Here we show that expression of olig2, which encodes a bHLH transcription factor, marks a distinct subset of neurons with similarities to eurydendroid neurons, the principal efferent neurons of the teleost cerebellum. In combination with other markers, olig2 reveals a dorsoventral organization of cerebellar neurons in embryos. Disruption of Hedgehog signaling, which patterns the ventral neural tube, produced a two-fold increase in the number of olig2(+) neurons. By contrast, olig2(+) neurons did not develop in embryos deficient for Wnt signaling, which patterns dorsal neural tube, nor did they develop in embryos deficient for both Hedgehog and Wnt signaling. Our data indicate that Hedgehog and Wnt work in opposit...Continue Reading

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Citations

Nov 11, 2009·PLoS Biology·Sandra RiegerReinhard W Köster
Apr 14, 2010·The Cerebellum·Samin A SajanKathleen J Millen
Jan 12, 2011·The European Journal of Neuroscience·Carmen G FeijóoVerónica A Palma
Mar 3, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Michael Schebesta, Fabrizio C Serluca
Mar 11, 2011·Annals of the New York Academy of Sciences·Yossy MachlufGil Levkowitz
May 5, 2017·Development, Growth & Differentiation·Masahiko HibiYasunori Murakami
Feb 11, 2011·Developmental Neurobiology·Masahiko Hibi, Takashi Shimizu
Jan 10, 2016·Cold Spring Harbor Perspectives in Biology·Julia Ganz, Michael Brand
Apr 18, 2009·Developmental Biology·Young-Ki BaeMasahiko Hibi
Apr 15, 2010·Developmental Biology·Shuichi KaniMasahiko Hibi
Nov 4, 2021·The Journal of Comparative Neurology·Takanori IkenagaMasahiko Hibi

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