Abstract
Objective: Primary infection with human herpes virus 6 (mainly HHV-6B) commonly occurs in the first 2 years of life leading to persistence and the possibility of virus reactivation later in life. Consequently, a specific cellular immune response is essential for effective control of virus reactivation. We have studied cell-mediated immune response to HHV-6 (U54) in healthy children and adolescents. Materials and Methods: By flow cytometry, the amount of cytokine (interferon gamma-IFN- γ, interleukin 2-IL-2, tumor necrosis factor alpha-TNF-α) secreting T-cells were measured after 10 days of pre-sensitization and 6 h of re-stimulation with mixtures of pooled overlapping peptides from U54, staphylococcal enterotoxin B (SEB, positive control), or Actin (negative control) in healthy children and adolescents without any underlying immune disorder or infectious disease. Results: All individuals showed a virus-specific response for at least one cytokine in either CD4+ or CD8+ cells. Percentages of individuals with HHV-6-specific TNF-α response in CD4+ (48% of individuals) as well as CD8+ (56% of individuals) were always the highest. Our data show significantly higher frequencies of HHV-6-specific TNF-α producing CD8+ T-cells in individ...Continue Reading
References
Jan 1, 1991·Microbiology and Immunology·Y YakushijinY Kobayashi
Apr 1, 1989·Journal of Clinical Microbiology·T OkunoK Baba
Oct 31, 1986·Science·S Z SalahuddinB Kramarsky
May 14, 1988·Lancet·K YamanishiT Kurata
Feb 1, 1993·Journal of Medical Virology·K N WardM J Sheldon
Oct 1, 1993·Journal of Virology·M YasukawaS Fujita
May 20, 1998·Viral Immunology·W KoideM Sakurai
Jul 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·V DardalhonN Taylor
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J Hassan, D J Reen
Jun 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sara JalecoNaomi Taylor
Nov 16, 2004·Journal of Medical Virology·L De BolleLieve Naesens
Feb 25, 2005·The New England Journal of Medicine·Danielle M ZerrLawrence Corey
Aug 3, 2005·The Journal of Experimental Medicine·Mark CobboldPaul A H Moss
Sep 9, 2005·The Journal of Experimental Medicine·Andrew W SylwesterLouis J Picker
May 16, 2008·Journal of Immunotherapy·Tobias FeuchtingerPeter Lang
Aug 25, 2009·Blood·Ann M LeenCatherine M Bollard
Jul 14, 2010·Blood·Tobias FeuchtingerHermann Einsele
Dec 8, 2010·Transfusion·Anita SchmittMichael Schmitt
Jan 18, 2011·Hematology/oncology Clinics of North America·Kevin Shiley, Emily Blumberg
Aug 16, 2011·The Journal of Pediatrics·Volker StrengerTherese Popow-Kraupp
Jul 19, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Michael UhlinJonas Mattsson
Nov 16, 2012·Blood·Ulrike GerdemannAnn M Leen
Jul 13, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P BarozziM Luppi