Hic-5 mediates the initiation of endothelial sprouting by regulating a key surface metalloproteinase

Journal of Cell Science
Jui M DaveKayla J Bayless

Abstract

During angiogenesis, endothelial cells must coordinate matrix proteolysis with migration. Here, we tested whether the focal adhesion scaffold protein Hic-5 (also known as TGFB1I1) regulated endothelial sprouting in three dimensions. Hic-5 silencing reduced endothelial sprouting and lumen formation, and sprouting defects were rescued by the return of Hic-5 expression. Pro-angiogenic factors enhanced colocalization and complex formation between membrane type-1 matrix metalloproteinase (MT1-MMP, also known as MMP14) and Hic-5, but not between paxillin and MT1-MMP. The LIM2 and LIM3 domains of Hic-5 were necessary and sufficient for Hic-5 to form a complex with MT1-MMP. The degree of interaction between MT1-MMP and Hic-5 and the localization of the complex within detergent-resistant membrane fractions were enhanced during endothelial sprouting, and Hic-5 depletion lowered the surface levels of MT1-MMP. In addition, we observed that loss of Hic-5 partially reduced complex formation between MT1-MMP and focal adhesion kinase (FAK, also known as PTK2), suggesting that Hic-5 bridges MT1-MMP and FAK. Finally, Hic-5 LIM2-LIM3 deletion mutants reduced sprout initiation. Hic-5, MT1-MMP and FAK colocalized in angiogenic vessels during porcin...Continue Reading

References

Dec 27, 1996·Cell·J Folkman, P A D'Amore
Dec 24, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·J L Guan
Oct 3, 1998·The Journal of Biological Chemistry·H FujitaK Tachibana
Oct 6, 1999·Current Opinion in Cell Biology·G Murphy, J Gavrilovic
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z ZhouK Tryggvason
Jul 11, 2000·Molecular Biology of the Cell·I Yana, S J Weiss
Sep 8, 2001·The Journal of Biological Chemistry·Y JiaW E Smoyer
Nov 27, 2001·Science·E CukiermanK M Yamada
Apr 9, 2002·Cell Structure and Function·Motoko ShibanumaKiyoshi Nose
May 7, 2002·Nature Reviews. Cancer·Mikala Egeblad, Zena Werb
Aug 3, 2002·Journal of Biochemistry·Naoyuki NishiyaKiyoshi Nose
Mar 13, 2003·Molecular Biology of the Cell·Motoko ShibanumaKiyoshi Nose
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Jul 8, 2003·Cell Motility and the Cytoskeleton·W Matthew Petroll, Lisha Ma
Feb 3, 2004·Journal of Cellular Biochemistry·Motoko ShibanumaKiyoshi Nose
May 12, 2004·Journal of Cellular Physiology·Pamela OsenkowskiRafael Fridman
Sep 24, 2004·Physiological Reviews·Michael C Brown, Christopher E Turner
Nov 17, 2004·The Journal of Cell Biology·Tae-Hwa ChunStephen J Weiss
Feb 17, 2005·Journal of Cell Science·Joo-ri Kim-KaneyamaMotoko Shibanuma
Dec 7, 2005·The Journal of Cell Biology·Ru Feng WuLance S Terada
Feb 14, 2006·Experimental Cell Research·Takahisa TakinoHiroshi Sato
Sep 26, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ruping WangDale D Tang
Feb 15, 2007·Journal of Cellular Physiology·David A Tumbarello, Christopher E Turner
Mar 6, 2007·American Journal of Physiology. Heart and Circulatory Physiology·C AvraamidesT S Panetti
May 25, 2007·Nature Reviews. Molecular Cell Biology·Ralf H Adams, Kari Alitalo
Dec 20, 2007·Cancer Research·Takahisa TakinoHiroshi Sato
Jul 25, 2008·Journal of Cell Science·Nicholas O Deakin, Christopher E Turner
Feb 17, 2009·Developmental Cell·M Luisa Iruela-Arispe, George E Davis

❮ Previous
Next ❯

Citations

Jan 13, 2018·Molecular Human Reproduction·Camille L DuranKayla J Bayless
Mar 21, 2019·Molecular Biology of the Cell·Anushree C GulvadyChristopher E Turner
Oct 15, 2020·American Journal of Physiology. Cell Physiology·Subhashree KumaravelSanjukta Chakraborty

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.