Hidden virulence determinants in a viral quasispecies in vivo.

Journal of Virology
Marta Sanz-RamosNoemí Sevilla

Abstract

The characterization of virulence determinants of pathogenic agents is of utmost relevance for the design of disease control strategies. So far, two classes of virulence determinants have been characterized for viral populations: those imprinted in the nucleotide sequence of some specific genomic regions and those that depend on the complexity of the viral population as such. Here we provide evidence of a virulence determinant that depends neither on a genomic sequence nor on detectable differences in population complexity. Foot-and-mouth disease virus is lethal for C57BL/6 mice showing the highest viral load in pancreas. Virus isolated from pancreas after one passage in mice showed an attenuated phenotype, with no lethality even at the highest dose tested. By contrast, virus from sera of the same mice displayed a virulence similar to that of the parental wild-type clone and virus isolated from spleen displayed an intermediate phenotype. However, viral populations from pancreas, spleen, and serum showed indistinguishable consensus genomic nucleotide sequences and mutant spectrum complexities, as quantified according to the mutation frequencies of both entire genomic nucleotide sequences of biological clones. The results show th...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·E DuarteJ Holland
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K M ChumakovI S Levenbook
Dec 1, 1990·Journal of Virology·J C de la Torre, J J Holland
May 1, 1988·The Journal of Experimental Medicine·R Ahmed, M B Oldstone
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B DietzscholdH Koprowski
Mar 26, 1982·Science·J HollandS VandePol
Jul 1, 1993·Scientific American·M Eigen
Sep 15, 1996·Nucleic Acids Research·J ClineH H Hogrefe
Nov 29, 1996·Journal of Molecular Biology·C EscarmísE Domingo
Jul 11, 1998·Journal of Virology·E BaranowskiE Domingo
Jun 25, 1999·AIDS Research and Human Retroviruses·P ShapshakK Goodkin
Sep 18, 2001·Journal of Virology·N ParienteE Domingo
Jan 16, 2003·Virus Research·Fred Brown
May 28, 2003·Journal of Virology·Nonia ParienteEsteban Domingo
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
May 1, 1964·Mutation Research·H J MULLER
Nov 1, 2003·Virology·Glòria SánchezRosa M Pintó
Mar 16, 2004·Journal of Virology·Claudia González-LópezEsteban Domingo
Sep 16, 2004·Journal of Virology·Hongye Li, Marilyn J Roossinck
Oct 14, 2004·Journal of Virology·Juan García-ArriazaCristina Escarmís
Oct 13, 2005·PLoS Pathogens·Julie K Pfeiffer, Karla Kirkegaard
Mar 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·Julie K Pfeiffer, Karla Kirkegaard
Mar 30, 2006·Current Topics in Microbiology and Immunology·E DomingoA Arias
Mar 30, 2006·Current Topics in Microbiology and Immunology·C EscarmísS C Manrubia
Aug 17, 2006·Journal of Virology·Akhtar AliMarilyn J Roossinck

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Citations

Nov 18, 2010·Bulletin of Mathematical Biology·Edgar Delgado-EckertNiko Beerenwinkel
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Samuel OjosnegrosEsteban Domingo
Apr 20, 2012·Journal of the Royal Society, Interface·Samuel OjosnegrosNiko Beerenwinkel
May 6, 2011·Journal of Virology·Christian J SauderSteven A Rubin
Jul 29, 2011·Journal of Virology·Karen Z Lancaster, Julie K Pfeiffer
Feb 4, 2011·Journal of Virology·Adam S Lauring, Raul Andino
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Esteban DomingoCelia Perales
Mar 24, 2012·Current Opinion in Virology·Antonio V BorderíaMarco Vignuzzi
Feb 16, 2010·Journal of Molecular Biology·Antonio MásMiguel Angel Martínez
Jun 5, 2012·Current Opinion in Microbiology·Karen Z Lancaster, Julie K Pfeiffer
Jan 25, 2011·Journal of Molecular Biology·Celia PeralesEsteban Domingo
Jun 29, 2010·Antiviral Research·Paula MolinariOscar Taboga
Jul 17, 2013·Virus Research·Sara Sadeghipour, Peter C McMinn
Feb 6, 2018·European Biophysics Journal : EBJ·Esteban Domingo, Celia Perales
Feb 20, 2010·Veterinary Research·Esteban Domingo
Sep 30, 2021·Annual Review of Virology·Esteban DomingoCelia Perales

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