Hierarchical map of protein unfolding and refolding at thermal equilibrium revealed by wide-angle X-ray scattering

Biochemistry
M HiraiKatsuaki Inoue

Abstract

Hierarchical features of the thermal unfolding-refolding structural transition of hen egg white lysozyme (HEWL) have been studied in the temperature range from 13 to 84 degrees C by using high-resolution wide-angle X-ray scattering (WAXS) measurements at a third-generation synchrotron source. We have gathered high-statistic WAXS data of the reversible unfolding-refolding process of HEWL in the q range from approximately 0.05 to approximately 3 A(-1) [q = (4pi/lambda) sin(theta/2), where theta is the scattering angle and lambda the wavelength]. This measured q range corresponds to the spatial distance from approximately 2 to approximately 125 A, which covers all hierarchical structures of a small globular protein such as HEWL, namely, tertiary, domain, and secondary structures. Because of this, we have found that the pH dependence of the thermal structural transition of HEWL is well characterized by the various hierarchical levels and the transition concurrence among them. In this report, we present a new hierarchical map depiction of unfolding-refolding transitions. Using scattering with various ranges of q values, we determine the molar ratio of native-like protein structure defined by the data in each range, thus producing a ...Continue Reading

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Citations

Jan 6, 2010·Journal of Structural and Functional Genomics·Lee Makowski
Oct 15, 2013·Journal of Synchrotron Radiation·Mitsuhiro HiraiNobutaka Shimuzu
Jul 16, 2013·The European Physical Journal. E, Soft Matter·Mitushiro HiraiGiuseppe Zaccai
Sep 13, 2008·Journal of Molecular Biology·Lee MakowskiRobert F Fischetti
Dec 1, 2005·Biochemical and Biophysical Research Communications·Sachio NaitoKeiji Wada
Aug 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Marina KatavaAlessandro Paciaroni
Sep 18, 2013·The Journal of Biological Chemistry·Zhaomin LiJian-Ting Zhang
Apr 19, 2019·Journal of Applied Crystallography·Amit SharmaRichard Neutze
Aug 13, 2010·The Journal of Physical Chemistry. B·Francesco D'AmicoClaudio Masciovecchio
Mar 1, 2012·The Journal of Physical Chemistry. B·Alessandro PaciaroniFrancesco Sacchetti
Nov 22, 2018·The Journal of Physical Chemistry. B·Mitsuhiro HiraiNobutaka Shimizu
Aug 28, 2018·The Journal of Physical Chemistry. B·Satoshi AjitoMitsuhiro Hirai

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