Hierarchical Micro-Nano Surface Topography Promotes Long-Term Maintenance of Undifferentiated Mouse Embryonic Stem Cells

Nano Letters
Mona JaggyPavel A Levkin

Abstract

Understanding of stem cell-surface interactions and, in particular, long-term maintenance of stem cell pluripotency on well-defined synthetic surfaces is crucial for fundamental research and biomedical applications of stem cells. Here, we show that synthetic surfaces possessing hierarchical micro-nano roughness (MN-surfaces) promote long-term self-renewal (>3 weeks) of mouse embryonic stem cells (mESCs) as monitored by the expression levels of the pluripotency markers octamer-binding transcription factor 4 (Oct4), Nanog, and alkaline phosphatase. On the contrary, culturing of mESCs on either smooth (S-) or nanorough polymer surfaces (N-surfaces) leads to their fast differentiation. Moreover, we show that regular passaging of mESCs on the hierarchical MN-polymer surface leads to an increased homogeneity and percentage of Oct4-positive stem cell colonies as compared to mESCs grown on fibroblast feeder cells. Immunostaining revealed the absence of focal adhesion markers on all polymer substrates studied. However, only the MN-surfaces elicited the formation of actin-positive cell protrusions, indicating an alternative anchorage mechanism involved in the maintenance of mESC stemness.

References

Nov 6, 1998·Science·J A ThomsonJ M Jones
Apr 5, 2000·Nature Biotechnology·B E ReubinoffA Bongso
Nov 25, 2000·Biology of Reproduction·N KirchhofH Niemann
Jan 9, 2001·Nature Cell Biology·C E Turner
Oct 3, 2001·Nature Biotechnology·C XuM K Carpenter
Aug 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kellar AutumnRobert J Full
Dec 5, 2003·Reproductive Biomedicine Online·Hossein Baharvand, Klaus Ingo Matthaei
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Oct 20, 2005·Journal of Bioscience and Bioengineering·Hiroshi MakinoYoshihiro Ito
Dec 22, 2006·PloS One·Masato NagaokaToshihiro Akaike
Aug 25, 2007·Stem Cells·Yohei HayashiMakoto Asashima
Apr 17, 2008·The International Journal of Developmental Biology·Livia EiselleovaPetr Dvorak
Jun 17, 2008·Nature Methods·Kristin AlbertiPeter W Zandstra
Aug 5, 2008·Biochemical and Biophysical Research Communications·Takamichi MiyazakiHirofumi Suemori
Jun 6, 2009·Advances in Biochemical Engineering/biotechnology·Michal Amit, Joseph Itskovitz-Eldor
Feb 18, 2010·Advanced Functional Materials·Pavel A LevkinJean M J Frechet
Jun 1, 2010·Nature Biotechnology·Luis G Villa-DiazGary D Smith
Jun 20, 2012·Experimental Cell Research·Geoffrey C HuntJean E Schwarzbauer
Oct 24, 2012·Journal of Biomedicine & Biotechnology·Michele M NavaRiccardo Pietrabissa
Jan 12, 2013·Nature Protocols·Mercè MartíJuan Carlos Izpisua Belmonte
Feb 8, 2014·Chemical Society Reviews·Xueli Liu, Shutao Wang
Jan 1, 2011·Genes·Francesca Soncin, Christopher M Ward
May 23, 2014·Nature Materials·William L MurphyAdam J Engler
May 23, 2014·Nature Materials·Matthew J DalbyRichard O C Oreffo
Jul 30, 2014·Nature Reviews. Genetics·Jared L SterneckertHans R Schöler
Feb 14, 2015·Nature Communications·Joseph RobertsonMartin J Humphries
Jul 30, 2015·Advanced Healthcare Materials·Yan ZhangWeiqiang Chen

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Citations

Feb 20, 2018·Tissue Engineering. Part B, Reviews·Sarita KumariDeepa Subramanyam
Apr 7, 2018·Biomaterials Science·Chara SimitziEmmanuel Stratakis
May 10, 2017·Biofabrication·Ch YiannakouE Stratakis
Jan 13, 2018·Tissue Engineering. Part B, Reviews·Xiaofang ChenYubo Fan
Nov 6, 2018·Cellular and Molecular Life Sciences : CMLS·Jiayun HuangWeiliang Shen
Mar 25, 2018·Advanced Materials·Wenqian FengPavel A Levkin
May 30, 2020·Development·David A Cruz Walma, Kenneth M Yamada
Mar 3, 2020·Frontiers in Cell and Developmental Biology·Sarah W ChanEvelyn K F Yim
Apr 13, 2020·Methods : a Companion to Methods in Enzymology·Steven Vermeulen, Jan de Boer
Jun 20, 2020·Colloids and Surfaces. B, Biointerfaces·Ali BabaiePeter Kingshott
Mar 24, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Robin AugustinePablo Maureira
Apr 6, 2018·ACS Applied Materials & Interfaces·Vito ErricoCesare Gargioli
Aug 10, 2021·APL Bioengineering·Linan CuiEvelyn K F Yim
Jan 14, 2019·ACS Biomaterials Science & Engineering·Lakshmi Kavitha SthanamShamik Sen
Dec 30, 2017·ACS Applied Materials & Interfaces·Jae-Hyeok LeeSangdun Choi
Jul 3, 2021·Chemical Reviews·Farhad SoheilmoghaddamJustin Cooper-White

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