Hierarchical roles of mitochondrial Papi and Zucchini in Bombyx germline piRNA biogenesis

Nature
Kazumichi M NishidaMikiko C Siomi

Abstract

PIWI-interacting RNAs (piRNAs) are small regulatory RNAs that bind to PIWI proteins to control transposons and maintain genome integrity in animal germ lines. piRNA 3' end formation in the silkworm Bombyx mori has been shown to be mediated by the 3'-to-5' exonuclease Trimmer (Trim; known as PNLDC1 in mammals), and piRNA intermediates are bound with PIWI anchored onto mitochondrial Tudor domain protein Papi. However, it remains unclear whether the Zucchini (Zuc) endonuclease and Nibbler (Nbr) 3'-to-5' exonuclease, both of which have pivotal roles in piRNA biogenesis in Drosophila, are required for piRNA processing in other species. Here we show that the loss of Zuc in Bombyx had no effect on the levels of Trim and Nbr, but resulted in the aberrant accumulation of piRNA intermediates within the Papi complex, and that these were processed to form mature piRNAs by recombinant Zuc. Papi exerted its RNA-binding activity only when bound with PIWI and phosphorylated, suggesting that complex assembly involves a hierarchical process. Both the 5' and 3' ends of piRNA intermediates within the Papi complex showed hallmarks of PIWI 'slicer' activity, yet no phasing pattern was observed in mature piRNAs. The loss of Zuc did not affect the 5'-...Continue Reading

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Citations

Jun 12, 2020·Biology of Reproduction·Deqiang Ding, Chen Chen
Jan 31, 2020·Nature·Natsuko IzumiYukihide Tomari
Jun 12, 2019·Wiley Interdisciplinary Reviews. RNA·Anna KolliopoulouLuc Swevers
Jan 15, 2020·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Kaoru Sato, Mikiko C Siomi
Sep 24, 2020·Cell Discovery·Shuqing ChenYongping Huang
Nov 18, 2018·Nature Reviews. Genetics·Deniz M OzataPhillip D Zamore
Feb 18, 2021·Genome Biology and Evolution·Caiti Smukowski HeilMaitreya J Dunham

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