Hierarchically aligned fibrin nanofiber hydrogel accelerated axonal regrowth and locomotor function recovery in rat spinal cord injury

International Journal of Nanomedicine
Shenglian YaoXiu-Mei Wang

Abstract

Designing novel biomaterials that incorporate or mimic the functions of extracellular matrix to deliver precise regulatory signals for tissue regeneration is the focus of current intensive research efforts in tissue engineering and regenerative medicine. To mimic the natural environment of the spinal cord tissue, a three-dimensional hierarchically aligned fibrin hydrogel (AFG) with oriented topography and soft stiffness has been fabricated by electrospinning and a concurrent molecular self-assembling process. In this study, the AFG was implanted into a rat dorsal hemisected spinal cord injury model to bridge the lesion site. Host cells invaded promptly along the aligned fibrin hydrogels to form aligned tissue cables in the first week, and then were followed by axonal regrowth. At 4 weeks after the surgery, neurofilament (NF)-positive staining fibers were detected near the rostral end as well as the middle site of defect, which aligned along the tissue cables. Abundant NF- and GAP-43-positive staining indicated new axon regrowth in the oriented tissue cables, which penetrated throughout the lesion site in 8 weeks. Additionally, the abundant blood vessels marked with RECA-1 had reconstructed within the lesion site at 4 weeks afte...Continue Reading

Citations

May 14, 2020·F1000Research·Mohammad-Masoud ZavvarianMichael Fehlings
Apr 10, 2019·Neural Regeneration Research·Shuo LiuBin Wang
Nov 3, 2020·Tissue Engineering. Part B, Reviews·Rafaela ZimmermannPatricia Pranke
Oct 30, 2020·Frontiers in Bioengineering and Biotechnology·Wenrui QuMinfei Wu
Feb 3, 2019·Acta Biomaterialia·Qingzheng ZhangXuesi Chen
Nov 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nazanin GhaneAmirkianoosh Kiani
May 18, 2021·Bioactive Materials·Wen XueBin Duan
May 18, 2021·Frontiers in Physiology·Chun YaoBin Yu
Aug 10, 2021·APL Bioengineering·Linan CuiEvelyn K F Yim
Dec 16, 2020·ACS Biomaterials Science & Engineering·Walaa A AbbasNageh K Allam
Jan 19, 2021·ACS Biomaterials Science & Engineering·Renchuan YouMingzhong Li
Oct 23, 2021·Experimental and Therapeutic Medicine·Jian-Tao LiuHua-Nan Li

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transmission electron microscopy
fluorescence microscopy
confocal microscopy

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