HIF-1α-activated TM4SF1-AS1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by enhancing TM4SF1 expression.

Biochemical and Biophysical Research Communications
Zhi ZengQiuran Xu

Abstract

Long non-coding RNAs (lncRNAs) are essential drivers or suppressors in human hepatocellular carcinoma (HCC) by participating in controlling transcription, translation, mRNA stability, and protein degradation protein-protein interaction. TM4SF1-AS1 is recently identified as a tumor-promoting factor in lung cancer. Nevertheless, its function in HCC and related molecular mechanisms remain unknown. Here, our data indicated that either hypoxia or hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitor (DMOG) induced the upregulation of TM4SF1-AS1 in HCC cells. HIF-1α knockdown rather than HIF-2α silencing remarkably abrogated hypoxia-upregulated TM4SF1-AS1 expression. Furthermore, we confirmed the elevated expression of TM4SF1-AS1 in HCC tissue samples and cell lines. The silencing of TM4SF1-AS1 prominently inhibited the proliferative, migratory, and invasive abilities of HCC cells. TM4SF1-AS1 depletion significantly blocked hypoxia-enhanced Hep3B cell proliferation and mobility. Interfering TM4SF1-AS1 remarkably reduced TM4SF1 mRNA and protein levels in HCC cells. But TM4SF1-AS1 knockdown did not impact the stability of TM4SF1 mRNA. Hypoxia enhanced the expression of TM4SF1 mRNA, which was subsequently decreased by TM4SF1-AS1 k...Continue Reading

References

Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Apr 14, 2016·Cancer Cell·Adam M Schmitt, Howard Y Chang
Feb 8, 2018·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Rui CaiWeijun Pang
Mar 3, 2018·International Journal of Molecular Sciences·Manuela LanzafameSalvatore Piscuoglio
Oct 14, 2018·Experimental & Molecular Medicine·Carolina Méndez-BlancoJosé L Mauriz
May 31, 2019·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Geng QinGangqiao Zhou
Aug 20, 2019·Biochemical and Biophysical Research Communications·Xue YangDemao Yao
Dec 27, 2019·Journal of Cellular and Molecular Medicine·ChuanrRong ZhuWu Ji
Jan 20, 2020·Journal of Hepatology·Sandrine FaivreRichard S Finn
Feb 19, 2021·Journal of Experimental & Clinical Cancer Research : CR·Liang WangQingguang Liu
Mar 31, 2021·Clinical and Translational Medicine·Bowen YaoKangsheng Tu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.