HIF-1alpha reveals a binding activity to the promoter of iNOS gene after permanent middle cerebral artery occlusion

Journal of Neurochemistry
C MatroneL Annunziato

Abstract

Hypoxia inducible factor (HIF-1)-1alpha is a specific, oxygen-sensitive protein that regulates the activity of HIF-1, a transcriptional factor that increases after cerebral ischemia and may either promote or prevent neuronal survival. In this study to determine whether the inducible nitric oxide synthase (iNOS) gene containing the sequence of the hypoxia-responsive enhancer (HRE) was an HIF-1 target after cerebral ischemia induced by permanent middle cerebral artery occlusion (pMCAO), electrophoretic mobility shift assay (EMSA) and iNOS western blot analysis were performed in the ischemic core, in the area surrounding the infarct and in the hippocampus ipsilateral and contralateral to the lesion. In addition, both HIF-1alpha mRNA and protein expression were examined in the ischemic core, in the area surrounding the ischemic core and in the hippocampus ipsilateral to the insult. Our results revealed that pMCAO up-regulates iNOS protein in the ischemic core, in the area surrounding the ischemic core and in the hippocampus ipsilateral to the lesion, and that the activation of iNOS expression is mediated by HIF-1. Moreover, HIF-1alpha mRNA and protein levels increased in the ischemic core and in the hippocampus ipsilateral to the l...Continue Reading

Citations

Feb 3, 2012·Cellular and Molecular Neurobiology·Neetu SinghVikas Mishra
Oct 18, 2005·Journal of Biomedical Science·Doo-Youn ChoiKeon Wook Kang
Feb 4, 2012·Journal of Agricultural and Food Chemistry·Ting-Lin YenJoen-Rong Sheu
Jun 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·C MatroneP Calissano
Dec 25, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kolenkode B KannanRena Feinman
Mar 8, 2005·American Journal of Respiratory and Critical Care Medicine·Guanxia ZhanSigrid C Veasey
Feb 9, 2012·American Journal of Respiratory Cell and Molecular Biology·Tanveer AhmadAnurag Agrawal
Jan 29, 2013·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Mohammad BayatGholamreza Hassanzadeh
Apr 13, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Hee Jeong KongChang Hoon Lee
Nov 30, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Shibani S MukerjiAlison K Hall
Jun 13, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Li Juan LiXia Chu
Nov 15, 2006·Molecular and Cellular Neurosciences·Michaël Van HoeckeAlain G Beley
Jan 28, 2014·Journal of Molecular and Cellular Cardiology·Martin HelanY S Prakash
Apr 17, 2007·Molecular Cell·Fang LiChuan-Yuan Li
Apr 8, 2015·Breast Cancer Research : BCR·Sergio Granados-PrincipalJenny C Chang
Oct 9, 2004·Stroke; a Journal of Cerebral Circulation·Giuseppe PignataroLucio Annunziato
Feb 5, 2011·Stroke; a Journal of Cerebral Circulation·Valeria ValsecchiLucio Annunziato
Apr 23, 2005·Glia·Claire L GibsonSean P Murphy
Jun 23, 2009·Nature Reviews. Neuroscience·Lorenzo GalluzziGuido Kroemer
Aug 8, 2019·The Journal of Trauma and Acute Care Surgery·Zi-Sen ZhangLiang-Ming Liu
Aug 23, 2018·Frontiers in Neuroscience·Rossana SirabellaGiuseppe Pignataro
Mar 22, 2019·Scientific Reports·Ana M MalihaSven Schnichels
Nov 28, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Walaa Yehia AbdelzaherMina Ezzat Attya
Oct 25, 2007·Biochemical Society Transactions·S Murphy, C L Gibson
Jan 4, 2021·International Immunopharmacology·Iman O Sherif, Nora H Al-Shaalan
Dec 8, 2020·Oxidative Medicine and Cellular Longevity·Yang LiuYanfeng Wang
Mar 17, 2020·Cell Calcium·Luigi FormisanoLucio Annunziato
Mar 5, 2021·Frontiers in Cell and Developmental Biology·Adam BajgarTomáš Doležal
Feb 11, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Atish GhewareBhavana Prasher
Jun 29, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Zirong PanGuanhua Du

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