HIF prolyl hydroxylase inhibition increases cell viability and potentiates dopamine release in dopaminergic cells

Journal of Neurochemistry
Jens Leander JohansenKenneth Thirstrup

Abstract

Hypoxia-inducible factor (HIF) controls the expression of genes that adapts the cellular condition to accommodate oxidative stress. The potential beneficial effect of HIF up-regulation in ischemia has recently gained interest substantiated by the known HIF-regulation of erythropoietin and other hypoxia accommodating genes. So far the perspectives for HIF up-regulation has been focused on anemia and ischemia related diseases but little information is available about the relevance of HIF biology for neurodegenerative disease like Parkinson's disease. We therefore sought out to characterize the effect of HIF-up-regulation on survival and dopamine homeostasis in dopaminergic cells. We used a low molecular weight HIF prolyl hydroxylase (HPH) inhibitor and lentiviral based shRNA knockdown of HPH subtypes as molecular tools to increase HIF protein level and downstream HIF-regulated genes. We show that HIF induction results in protection against oxidative stress in cellular models based on PC12 cells and LUHMES cells. In addition, HPH inhibition elevates tyrosine hydroxylase expression and activity, which causes increased dopamine synthesis and release in both PC12 cells and a primary rat ventral mesencephalic cell culture. All togethe...Continue Reading

References

Aug 1, 1990·Journal of Neuroscience Research·P P Michel, F Hefti
Feb 10, 2000·European Journal of Pharmacology·I LamensdorfI J Kopin
Oct 13, 2001·Science·R K Bruick, S L McKnight
Feb 2, 2002·Science·David LandoMurray L Whitelaw
Sep 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mircea IvanWilliam G Kaelin
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anna V KuznetsovaMaria F Czyzyk-Krzeska
Apr 15, 2003·Journal of Applied Physiology·Ganesh K KumarNanduri R Prabhakar
Jul 31, 2003·The Journal of Biological Chemistry·Eric S HansonElizabeth A Leibold
Jan 30, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·G S RalphD Krige
May 21, 2004·Nature Reviews. Neuroscience·Frank R Sharp, Myriam Bernaudin
Jul 13, 2004·The Journal of Biological Chemistry·Rebecca J AppelhoffJonathan M Gleadle
Dec 1, 2004·Journal of Neurochemistry·Peter R DunkleyPhillip W Dickson
Jan 15, 2005·Biochemical and Biophysical Research Communications·Adrienne M GormanAfshin Samali
Jan 26, 2005·Molecular Biotechnology·Jens Leander JohansenPhilip Kusk
Jul 8, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Julie LothariusMarcel Leist
Oct 18, 2005·The Journal of Biological Chemistry·Ambreena SiddiqRajiv R Ratan
Jan 12, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Javorina MilosevicJohannes Schwarz
Sep 1, 2007·Journal of Neurochemistry·David J LombRobert S Freeman
May 13, 2008·Trends in Neurosciences·W Michael CaudleGary W Miller
Jan 22, 2009·Journal of Neuroscience Research·Louise WittenArne Mørk
Feb 27, 2010·Movement Disorders : Official Journal of the Movement Disorder Society·Bobby Thomas, M Flint Beal

❮ Previous
Next ❯

Citations

Oct 12, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nobuyuki FujitaMakarand V Risbud
Apr 21, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Kenneth ThirstrupHenrik Sindal Jensen
Feb 5, 2013·Free Radical Biology & Medicine·Orly WeinrebTamar Amit
Apr 30, 2013·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Ravi KantAmteshwar Singh Jaggi
Mar 8, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thamara E OsingaIdo P Kema
May 13, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zachary R SchoepflinMakarand V Risbud
Mar 19, 2016·Oncotarget·Sandeep Vasant More, Dong-Kug Choi
Feb 22, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nobuyuki FujitaMakarand V Risbud
Jan 16, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mehrnaz MehrabaniSolmaz Mirzamohammadi
Mar 28, 2020·Journal of Clinical Medicine·Yi-Chou HouYuh-Feng Lin
Feb 15, 2018·Journal of Alzheimer's Disease : JAD·Niraj Kumar JhaPravir Kumar
Aug 17, 2021·Frontiers in Cell and Developmental Biology·Elena V MitroshinaMaria V Vedunova
Aug 28, 2021·Biology·Laura Lestón PinillaLuigi A De Girolamo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Amygdala and Midbrain Dopamine

The midbrain dopamine system is widely studied for its involvement in emotional and motivational behavior. Some of these neurons receive information from the amygdala and project throughout the cortex. When the circuit and transmission of dopamine is disrupted symptoms may present. Here is the latest research on the amygdala and midbrain dopamine.