HIF1α-AS1 is a DNA:DNA:RNA triplex-forming lncRNA interacting with the HUSH complex

BioRxiv : the Preprint Server for Biology
M. S. LeisegangRalf P. Brandes

Abstract

DNA:DNA:RNA triplexes that are formed through Hoogsteen base-pairing have been observed in vitro, but the extent to which these interactions occur in cells and how they impact cellular functions remains elusive. Using a combination of bioinformatic techniques, RNA/DNA pulldown and biophysical studies, we set out to identify functionally important DNA:DNA:RNA triplex-forming long non-coding RNAs (lncRNA) in human endothelial cells. The lncRNA HIF1-AS1 was retrieved as a top hit. Endogenous HIF1-AS1 reduced the expression of numerous genes, including EPH Receptor A2 and Adrenomedullin through DNA:DNA:RNA triplex formation by acting as an adapter for the repressive human silencing hub complex (HUSH). Moreover, the oxygen-sensitive HIF1-AS1 was down-regulated in pulmonary hypertension and loss-of-function approaches not only resulted in gene de-repression but also enhanced angiogenic capacity. As exemplified here with HIF1-AS1, DNA:DNA:RNA triplex formation is a functionally important mechanism of trans-acting gene expression control.

Datasets Mentioned

BETA
PXD023512

Methods Mentioned

BETA
transfection
immunoprecipitation
RIP
Assay
NMR
electrophoretic mobility shift assay
electrophoretic mobility shift
Circular
pulldown
ChIP

Software Mentioned

ENCODE
Ensembl
Triplex Domain Finder ( TDF
CPC2
MACS2
Triplex Domain Finder
R
phenoscanner
Coding Potential Assessment Tool ( CPAT ) potential calculator...
BLAT

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