HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5p.

Cell Death & Disease
Yufan ZhouZhiming Wang

Abstract

Hepatocellular carcinoma (HCC) remains the primary cause of cancer-related death. Metabolic change is the major characteristic of cancer. The present study attempted to investigate the regulatory mechanisms of HCC energy metabolism from the perspective of noncoding RNA regulation of HIF1A and LDHA. The expression of miR-100-5p expression was significantly suppressed in HCC tissue samples and HCC cell lines under 1% O2-induced hypoxia. miR-100-5p overexpression significantly suppressed hypoxia-induced increases in lactate concentration and glucose uptake. Exposure to 1% O2 induced HIF1A protein and reduced miR-100-5p expression, while HIF1A silencing dramatically rescued miR-100-5p expression upon 1% O2 exposure. In addition, 1% O2-induced increases in lactate concentration and glucose uptake were also suppressed by HIF1A silencing. Next, by analyzing available data in TCGA, we found that lncRNA RAET1K was correlated with HIF1A and miR-100-5p.LncRNA RAET1K could downregulate the expression of miR-100-5p by acting as a sponge, while HIF1A bound the lncRNA RAET1K promoter region to activate its transcription. LncRNA RAET1K silencing significantly suppressed HCC cell proliferation and invasion and also suppressed hypoxia-induced in...Continue Reading

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Citations

Aug 28, 2020·World Journal of Gastroenterology : WJG·Zhao-Shan NiuLi-Mei-Li Tian
Nov 25, 2020·Wiley Interdisciplinary Reviews. RNA·Joseph Eniafe, Shuai Jiang
Oct 31, 2020·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Mar 4, 2021·Cancers·Amar BalihodzicMartin Pichler
Apr 4, 2021·International Journal of Molecular Sciences·Lok-Sze Wong, Chun-Ming Wong
Apr 17, 2021·Biochemical and Biophysical Research Communications·Xiwu OuyangYao Xiao
Jul 22, 2021·Cell Biology International·Wanyuan RuanRunsang Pan

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Methods Mentioned

BETA
surgical resection
antisense oligonucleotides
PCR
transfections
fluorescence microscopy
transfection
xenograft
Assay
immunoprecipitation
ChIP

Software Mentioned

Jaspar
SPSS17

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