High- and low-mobility stages in the synaptic vesicle cycle.

Biophysical Journal
Dirk KaminSilvio O Rizzoli

Abstract

Synaptic vesicles need to be mobile to reach their release sites during synaptic activity. We investigated vesicle mobility throughout the synaptic vesicle cycle using both conventional and subdiffraction-resolution stimulated emission depletion fluorescence microscopy. Vesicle tracking revealed that recently endocytosed synaptic vesicles are highly mobile for a substantial time period after endocytosis. They later undergo a maturation process and integrate into vesicle clusters where they exhibit little mobility. Despite the differences in mobility, both recently endocytosed and mature vesicles are exchanged between synapses. Electrical stimulation does not seem to affect the mobility of the two types of vesicles. After exocytosis, the vesicle material is mobile in the plasma membrane, although the movement appears to be somewhat limited. Increasing the proportion of fused vesicles (by stimulating exocytosis while simultaneously blocking endocytosis) leads to substantially higher mobility. We conclude that both high- and low-mobility states are characteristic of synaptic vesicle movement.

References

Feb 1, 1992·The Journal of Cell Biology·M K BennettR H Scheller
Mar 1, 1981·The Journal of Cell Biology·J E Heuser, T S Reese
Feb 1, 1984·The Journal of Cell Biology·T M Miller, J E Heuser
Mar 1, 1994·The Journal of Cell Biology·W J Betz, A W Henkel
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S HilfikerP Greengard
Oct 24, 2001·Trends in Neurosciences·M A Cousin, P J Robinson
May 21, 2003·The Journal of Cell Biology·Ona BloomOleg Shupliakov
Jun 6, 2003·Nature·Sunil P Gandhi, Charles F Stevens
Mar 27, 2004·Science·Silvio O Rizzoli, William J Betz
Jun 26, 2004·Annual Review of Neuroscience·Thomas C Sudhof
Aug 23, 2005·Biophysical Journal·Matthew ShtrahmanXiao-Lun Wu
Nov 25, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Edward A Lemke, Jurgen Klingauf
Feb 8, 2006·Nature Neuroscience·Kevin J DarcyYukiko Goda
Nov 18, 2006·Cell·Shigeo TakamoriReinhard Jahn
Dec 14, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michael A Gaffield, William J Betz
Feb 23, 2008·Science·Volker WestphalStefan W Hell
Jun 3, 2008·Microscopy Research and Technique·Annedore PungeStefan W Hell
Mar 17, 2010·Traffic·Felipe OpazoSilvio O Rizzoli

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Citations

Jan 17, 2014·Journal of Chemical Biology·Jose Requejo-Isidro
Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Annette DenkerSilvio O Rizzoli
Jun 29, 2011·The Journal of General Physiology·Graham C R Ellis-Davies, Edward N Pugh
Oct 16, 2012·Experimental Neurology·Jan Tønnesen, U Valentin Nägerl
Sep 17, 2011·Molecular and Cellular Neurosciences·Harold D MacGillavryThomas A Blanpied
Mar 15, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sinem Saka, Silvio O Rizzoli
Dec 19, 2014·Synapse·Dario Maschi, Vitaly A Klyachko
Feb 3, 2015·ELife·Beyenech BinottiReinhard Jahn
Nov 5, 2016·The Journal of Cell Biology·Merja JoensuuFrédéric A Meunier
Dec 12, 2017·Frontiers in Cellular Neuroscience·Janosch P Heller, Dmitri A Rusakov
Aug 22, 2015·Nature Communications·Stephanie A ReyKevin Staras
Jun 26, 2015·The EMBO Journal·Jerome Di Giovanni, Zu-Hang Sheng
May 2, 2020·Journal of Neurochemistry·Suzanne O NolanErin S Calipari
Jul 7, 2020·The EMBO Journal·Sofiia ReshetniakSilvio O Rizzoli
Oct 13, 2016·The Biochemical Journal·Manuel MaidornFelipe Opazo
Feb 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Chungwon ParkHyokeun Park
Feb 6, 2021·Journal of Neurochemistry·Callista B Harper, Karen J Smillie

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