PMID: 45528Jan 1, 1977

High activity of NADP-dependent malic enzyme in mitochondria from abdomen muscle of the crayfish Orconectes limosus

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
E F SkorkowskiZ Aleksandrowicz

Abstract

1. Mitochondria isolated from abdomen muscle of crayfish Orconectes limosus exhibit malic enzyme activity in the presence of L-malate, NADP and Mn2+ ions after addition of Triton X-100. Under optimal conditions about 230 nmole of reduced NADP and an equivalent amount of pyruvate are produced per min per mg of mitochondrial protein. 2. The pH optimum for decarboxylation of L-malate is about 7.5. 3. The apparent Km for L-malate, NADP and Mn2+ ions was found to be 0.66, 0.012, and 0.0025 mM, respectively. 4. The requirement for Mn2+ can be replaced by Mg2+, Co2+ and Ni2+ ions; however, higher concentrations of these ions than Mn2+ are required for a full stimulation of malic enzyme activity. 5. Oxaloacetate and pyruvate inhibited the enzyme activity in a competitive manner with apparent Ki values of 0.05 mM and 5.4 mM, respectively.

References

Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E F Skorkowski, J Swierczyński
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E F SkorkowskiJ Swierczyński
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A Biegniewska, E F Skorkowski
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E F Skorkowski
Nov 1, 1991·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·R LuchettiO Soragni
May 22, 2019·Fish Physiology and Biochemistry·J GronczewskaE F Skorkowski

Citations

Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E F SkorkowskiJ Swierczyński
Dec 18, 1975·Biochimica Et Biophysica Acta·F WadaY Sakamoto
Apr 5, 1968·Biochemical and Biophysical Research Communications·E R SimpsonR W Estabrook
Sep 1, 1972·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K BartholoméD Pette
Nov 27, 1974·Biochimica Et Biophysica Acta·J Swierczyński, Z Aleksandrowicz
Sep 1, 1972·Archives of Biochemistry and Biophysics·R Frenkel
Apr 30, 1971·European Journal of Biochemistry·D Brdiczka, D Pette
Jan 1, 1964·Advances in Enzyme Regulation·H A LardyP D Ray

Related Concepts

Malate dehydrogenase (NADP)
Malate Dehydrogenase
Mitochondria
ME1
Abdominal Muscles
Procambarus
Hydrogen-Ion Concentration
Pyruvates

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