High-affinity and undissociated capillary electrophoresis for DNA strand exchange analysis.

Chemical Communications : Chem Comm
Fangzhi YuHailin Wang

Abstract

By identification of a super-stable protein-DNA-affinity system, we developed a free-solution capillary electrophoresis approach for rapid and sensitive detection of fundamentally important DNA strand exchange reactions mediated by recombinases. We further extended this assay for identification of hyper-recombinases generated from bioengineering and detection of single DNA mismatches caused by replication error.

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Methods Mentioned

BETA
electrophoresis

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