PMID: 9191981Feb 1, 1997Paper

High-affinity binding of corticosterone to mammalian neuronal membranes: possible role of corticosteroid binding globulin

The Journal of Steroid Biochemistry and Molecular Biology
M OrchinikB S McEwen

Abstract

The signal transduction mechanisms mediating rapid steroid actions are poorly understood. To characterize corticosteroid interaction with neuronal membranes in a species with rapid behavioral responses to corticosterone, we examined [3H]corticosterone binding to membranes prepared from prairie vole brains. At 22 degrees C, the rates of association and dissociation of [3H]corticosterone with well-washed synaptosomal membranes were very rapid. Specific binding was characterized by high affinity (Kd = 6.01 nM) and low density (Bmax = 63.1 fmol/mg protein). The binding sites were highly specific for naturally occurring glucocorticoids and the density of binding sites appeared to vary by neuroanatomical region. Unlike most G-protein-coupled receptors, the high-affinity binding of [3H]corticosterone to vole brain membranes was unaffected by the addition of Mg2+ or guanyl nucleotides. Surprisingly, saline perfusion of vole brains before tissue homogenization greatly reduced high-affinity binding. In addition, the affinity and specificity of corticosteroid binding sites were similar in vole neuronal membranes and vole plasma. These data suggest that corticosteroid binding globulins may facilitate [3H]corticosterone binding to neuronal ...Continue Reading

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Citations

Feb 24, 2011·Oecologia·Michael J SheriffRudy Boonstra
Apr 12, 2001·Journal of Neurochemistry·N SchneiderM Goppelt-Struebe
Mar 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Matthew N Hill, Bruce S McEwen
Feb 26, 2010·Molecular Brain·Therese RiedemannOsborne F X Almeida
Aug 3, 2001·General and Comparative Endocrinology·P A Schiml-WebbE F Rissman
Jul 13, 2000·General and Comparative Endocrinology·M OrchinikP J Gasser
Jan 3, 2013·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Elena SivukhinaGustav F Jirikowski
Oct 8, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Rene F ChunMartin Hewison
Dec 21, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L Michael RomeroJohn C Wingfield
Feb 7, 2021·General and Comparative Endocrinology·Michelle A Rensel, Barney A Schlinger
Sep 22, 2012·The Journal of Experimental Biology·Elena SivukhinaMarie-Pierre Moisan

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