High affinity binding of latent matrix metalloproteinase-9 to the alpha2(IV) chain of collagen IV.

The Journal of Biological Chemistry
M W OlsonR Fridman

Abstract

Association of matrix metalloproteinases (MMPs) with the cell surface and with areas of cell-matrix contacts is critical for extracellular matrix degradation. Previously, we showed the surface association of pro-MMP-9 in human breast epithelial MCF10A cells. Here, we have characterized the binding parameters of pro-MMP-9 and show that the enzyme binds with high affinity (Kd approximately 22 nM) to MCF10A cells and other cell lines. Binding of pro-MMP-9 to MCF10A cells does not result in zymogen activation and is not followed by ligand internalization, even after complex formation with tissue inhibitor of metalloproteinase-1 (TIMP-1). A 190-kDa cell surface protein was identified by ligand blot analysis and affinity purification with immobilized pro-MMP-9. Microsequencing and immunoblot analysis revealed that the 190-kDa protein is the alpha2(IV) chain of collagen IV. Specific pro-MMP-9 surface binding was competed with purified alpha2(IV) and was significantly reduced after treatment of the cells with active MMP-9 before the binding assay since alpha2(IV) is hydrolyzed by MMP-9. A pro-MMP-9.TIMP-1 complex and MMP-9 bind to alpha2(IV), suggesting that neither the C-terminal nor the N-terminal domain of the enzyme is directly inv...Continue Reading

References

Oct 1, 1992·Current Opinion in Cell Biology·W T Chen
Dec 1, 1990·Cancer Metastasis Reviews·J E Testa, J P Quigley
Apr 1, 1990·Trends in Genetics : TIG·L M Matrisian
Jun 15, 1990·International Journal of Cancer. Journal International Du Cancer·S ZuckerJ W Schwedes
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Jul 1, 1995·The Biochemical Journal·J A AllanG Murphy
Jan 1, 1995·Methods in Enzymology·G Murphy, T Crabbe
Nov 1, 1994·International Journal of Cancer. Journal International Du Cancer·D W VisscherR Fridman
Feb 15, 1994·International Journal of Cancer. Journal International Du Cancer·M HöyhtyäC M Liang
Feb 1, 1993·The Journal of Investigative Dermatology·T V KarelinaA Z Eisen
Oct 1, 1995·Current Opinion in Cell Biology·H Birkedal-Hansen

❮ Previous
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Citations

Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S M Ellerbroek, M S Stack
May 9, 2006·Cancer Metastasis Reviews·Rosemarie ChircoHyeong-Reh Choi Kim
Aug 7, 2003·Biochemical and Biophysical Research Communications·Marta TothRafael Fridman
Jul 20, 2002·Journal of Molecular Biology·Hyunju ChaHans Brandstetter
Oct 12, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·A C WheatcroftG S Evans
Sep 17, 2002·Current Opinion in Cell Biology·Motoharu Seiki
Oct 6, 1999·Current Opinion in Cell Biology·G Murphy, J Gavrilovic
May 10, 2001·Drug Discovery Today·H D. Foda, S Zucker
Jan 18, 2012·Journal of Medicinal Chemistry·Jun WangJohn F Gilmer
Dec 21, 2002·Differentiation; Research in Biological Diversity·Conor C Lynch, Lynn M Matrisian
Sep 15, 2010·The Journal of Biological Chemistry·Antoine DufourJian Cao
Jul 4, 2008·Molecular & Cellular Proteomics : MCP·Danmei XuBenedikt M Kessler
Jan 30, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Nese KaradagVedat Kirimlioglu
Jul 23, 1999·Annals of the New York Academy of Sciences·V DoloM L Vittorelli
Nov 1, 2001·Annual Review of Cell and Developmental Biology·M D Sternlicht, Z Werb
Oct 10, 2006·Nephron. Experimental Nephrology·Takayuki KurodaToshihiro Shinosaki
Nov 9, 2004·American Journal of Respiratory and Critical Care Medicine·Hitesh S DeshmukhGeorge D Leikauf
Jun 13, 2003·Genome Biology·Robert P T SomervilleSuneel S Apte
Nov 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A MizutaniN Tsukagoshi
Apr 21, 2009·Future Oncology·Laurie G HudsonM Sharon Stack
Jul 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Melanie RolliBrunhilde Felding-Habermann
May 3, 2016·Veterinary and Comparative Oncology·L H Pulz, R F Strefezzi
Jun 26, 2003·Veterinary Pathology·P LoukopoulosW F Robinson
Jul 19, 2002·Journal of Cellular Physiology·Serenella M PupaElda Tagliabue
May 29, 2002·Pathology International·Kazuki NabeshimaTetsuro Sameshima
Feb 13, 2002·The Journal of Biological Chemistry·Gregory A BannikovGregory I Goldberg
Sep 2, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nagako YoshibaHervé Lesot
Jul 8, 2003·The Journal of Pathology·Ivan Stamenkovic
Apr 20, 2006·Birth Defects Research. Part C, Embryo Today : Reviews·Michael N VanSaun, Lynn M Matrisian
Oct 21, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Thomas Ludwig

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