PMID: 8455021Apr 1, 1993Paper

High-affinity choline transport sites: use of [3H]hemicholinium-3 as a quantitative marker

Journal of Neurochemistry
H K Happe, L C Murrin

Abstract

High-affinity choline transport (HAChT), the rate-limiting and regulatory step in acetylcholine (ACh) synthesis, is selectively localized to cholinergic neurons. Hemicholinium-3 (HC3), a potent and selective inhibitor of HAChT, has been used as a specific radioligand to quantify HAChT sites in membrane binding and autoradiographic studies. Because both HAChT velocity and [3H]HC3 binding change as in vivo activity of cholinergic neurons is altered, these markers are also useful measures of cholinergic neuronal activity. Evidence that [3H]HC3 is a specific ligand for HAChT sites on cholinergic terminals is reviewed. The ion requirements of HAChT and [3H]HC3 binding indicate that sodium and chloride are required for recognition of both choline and [3H]HC3. A common recognition site is also indicated by the close correspondence of the potency of HC3 and choline analogues for inhibiting both HAChT and [3H]HC3 binding. The parallel regional distributions of both markers in adult brain, during development and after specific lesions, all indicate specific cholinergic localization. The close association of HAChT and [3H]HC3 binding sites is also supported by parallel regulatory changes occurring after in vivo drug treatments and in vitr...Continue Reading

References

Jan 1, 1978·Journal of Neurochemistry·M J Kuhar, L C Murrin
Jul 1, 1976·Journal of Neurochemistry·J R Simon, M J Kuhar
May 15, 1975·Biochemical Pharmacology·J R SimonJ M Kuhar
Jan 1, 1975·Journal of Neural Transmission·L L ButcherL Bilezikjian
Jul 1, 1992·Trends in Neurosciences·G R Uhl
Jun 18, 1991·Biochimica Et Biophysica Acta·M KnipperH Breer
Dec 1, 1990·Brain Research Bulletin·V L DawsonJ K Wamsley
Mar 1, 1989·Veterinary Pathology·H S Singer, L C Cork
Feb 27, 1989·Brain Research·J W Bekenstein, G F Wooten
Mar 13, 1989·FEBS Letters·M KnipperH Breer
Nov 15, 1988·Biochemical Pharmacology·K YamadaJ T Coyle
Mar 3, 1987·European Journal of Pharmacology·M D SaltarelliJ T Coyle
Dec 4, 1987·Neuroscience Letters·G F HohmannJ T Coyle
Aug 27, 1986·Brain Research·P R Lowenstein, J T Coyle

❮ Previous
Next ❯

Citations

Jan 1, 1994·Journal of Neural Transmission. Parkinson's Disease and Dementia Section·R Rodríguez-PuertasJ Pascual
Jul 5, 1996·Mechanisms of Ageing and Development·T ArakiY Itoyama
Jun 1, 1995·Neurochemistry International·A WawrzeńczykM J Nałecz
Mar 1, 1995·Neurobiology of Aging·T D SmithF M Leslie
Jul 4, 1997·Brain Research·V VogelsbergM Hadjiconstantinou
Feb 1, 1997·Brain Research. Brain Research Reviews·M Sarter, J P Bruno
Feb 1, 1997·Neurochemistry International·Y IkarashiY Maruyama
Dec 20, 1996·Environmental Toxicology and Pharmacology·T ArakiY Itoyama
Nov 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lingyan ZhangJohn Lew
Apr 16, 2010·The Journal of Biological Chemistry·Lorise C GahringScott W Rogers
Jan 1, 1994·Journal of Pineal Research·M SamejimaM Ebadi
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·J AraH Ischiropoulos
Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y DuS M Paul
Jul 31, 2014·Journal of Biochemistry·Tatsuya Haga
Mar 10, 2006·Journal of Neurochemistry·Fabiola M RibeiroMarco A M Prado
Jan 29, 2000·Nature Neuroscience·T OkudaI Katsura
Jul 2, 2008·Annals of the New York Academy of Sciences·Vinay Parikh, Martin Sarter
Jun 12, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Yael Abreu-VillaçaAnderson Ribeiro-Carvalho
Sep 13, 2001·The Journal of Biological Chemistry·D H SweetJ B Pritchard
Jun 11, 1999·The American Journal of Physiology·A R VillalobosJ L Renfro
Sep 19, 2002·The Journal of Biological Chemistry·Takashi OkudaDavid Gurwitz
Jan 18, 2005·The Journal of Biological Chemistry·In Sup Kil, Jeen-Woo Park
Mar 1, 2021·European Journal of Pharmacology·Takashi OkudaHidemi Misawa
Feb 12, 1998·Biochemical and Biophysical Research Communications·J E MroczkowskaK A Nalecz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.