High affinity Concanavalin A binding to sterol-depleted L cells

Journal of Cellular Physiology
J D Marshall, H J Heiniger

Abstract

Binding of Concanavalin A to mouse L cells which were grown in serum free, chemically defined medium and depleted of their membrane sterol by blocking their de novo sterol synthesis was investigated. Kinetic analysis of binding data implied positive cooperativity, with two different affinities for Con A, in both experimental and control cultures. The amount of Con A bound to the cell surface at saturation was approximately 0.5 picomoles per mg cellular protein in controls and approximately 1.0 picomoles per mg cellular protein in 25-hydroxycholesterol treated cultures (which had a reduced sterol concentration of up to 50% in their plasma membranes). This phenomenon was reversed when cholesterol or mevalonate was added to the inhibited cultures to compensate for their inability to synthesize sterol. Our findings indicate that lectin binding to specific glycoprotein receptors is influenced by membrane lipid composition.

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Citations

Mar 1, 1981·Experimental Cell Research·M De Felici, G Siracusa
Sep 1, 1981·Journal of Cellular Physiology·W A MalteseJ J Volpe
Aug 1, 1984·Journal of Cellular Physiology·G A Boissonneault, H J Heiniger

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