High affinity, dsRNA binding by disconnected interacting protein 1.

Biochemical and Biophysical Research Communications
Daniel J Catanese, K S Matthews

Abstract

Disconnected interacting protein 1 (DIP1) appears from sequence analysis and preliminary binding studies to be a member of the dsRNA-binding protein family. Of interest, DIP1 was shown previously to interact with and influence multiple proteins involved in transcription regulation in Drosophila melanogaster. We show here that the longest isoform of this protein, DIP1-c, exhibits a 500-fold preference for dsRNA over dsDNA of similar nucleotide sequence. Further, DIP1-c demonstrated very high affinity for a subset of dsRNA ligands, with binding in the picomolar range for VA1 RNA and miR-iab-4 precursor stem-loop, a potential physiological RNA target involved in regulating expression of its protein partner, Ultrabithorax.

References

Nov 1, 1992·Molecular and Cellular Biology·L MancheM B Mathews
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D St JohnstonM Jantsch
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S CumberledgeS Sakonju
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
Jan 1, 1995·Molecular and Cellular Biology·S R GreenM B Mathews
Mar 4, 2000·The EMBO Journal·A RamosG Varani
Dec 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Esther BaeRobert A Drewell
May 30, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Laura R Saunders, Glen N Barber
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Aug 16, 2003·Developmental Cell·Alexei A AravinThomas Tuschl
Apr 24, 2004·Science·Soraya YektaDavid P Bartel
Sep 11, 2004·FEBS Letters·Edward G HittiMichael F Jantsch
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·Bin TianMichael B Mathews
Apr 28, 2005·The FEBS Journal·Kung-Yao Chang, Andres Ramos
Dec 17, 2005·Genes & Development·Matthew RonshaugenEric C Lai
Feb 14, 2006·Structure·Richard SteflFrédéric H-T Allain
Jul 5, 2008·Journal of Molecular Biology·Peter A LemaireJames L Cole
Jul 16, 2008·FEBS Letters·Shaun J CurtinPeter M Waterhouse
May 19, 2009·Journal of Molecular Biology·Laurie A HeinickePhilip C Bevilacqua

❮ Previous
Next ❯

Citations

Mar 17, 2015·Biochemical and Biophysical Research Communications·Qi ZhangQingfa Wu
Oct 6, 2011·Biochemical and Biophysical Research Communications·Daniel J Catanese, Kathleen S Matthews
Oct 4, 2017·Nature Communications·Jing Ting WongJun Wei Pek

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.