Mar 18, 2010

High-affinity folate receptor in cardiac neural crest migration: a gene knockdown model using siRNA

Developmental Dynamics : an Official Publication of the American Association of Anatomists
T H RosenquistG D Bennett

Abstract

Folate supplementation reduces the incidence of congenital heart defects, but the nature of this protective mechanism remains unclear. Immunolabeling demonstrated that the neural tube and neural crest (NC) cells were rich in the high-affinity folate receptor FOLR1and during the early stages of development FOLR1 was found principally in these cells. Suppression of Folr1 expression in the nascent cardiac NC by site-directed short-interfering RNA (siRNA) altered cardiac NC cell mitosis and subsequent migration patterns leading to abnormal development of the pharyngeal arch arteries (PAA) and outflow tract. qPCR analysis demonstrated that the siRNA treatment significantly reduced Folr1 24 hr after treatment. These treatments also significantly reduced mitosis in the neural tube, but adjacent, nontreated areas were unaffected. In summary, a brief reduction in the expression of Folr1 during a critical stage of NC development had long-term consequences for the development of the PAA and outflow tract.

  • References52
  • Citations6
  • References52
  • Citations6

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Arterial System
Neural Tube
Gene Knockdown Techniques
RNA, Small Interfering
Cell Motility
Hormone Receptors, Cell Surface
Folate Receptor 1
Folate receptor
Folic acid supplementation

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