PMID: 9443935Jan 28, 1998Paper

High affinity glutamate transport in rat cortical neurons in culture

Molecular Pharmacology
G J WangPaul A Rosenberg

Abstract

We assayed glutamate transport activity in cultures of rat cortical neurons containing < 0.2% astrocytes. Using [3H]L-glutamate as the tracer, sodium-dependent high affinity glutamate transport was demonstrated [K(m) = 17.2 +/- 2.4 microM; Vmax = 3.3 +/- 0.32 nmol/mg of protein/min (n = 5)]. Dihydrokainate (1 mM) inhibited uptake of radioactivity by 88 +/- 3% and had a Ki value of 65 +/- 7 microM. L-alpha-Aminoadipate (1 mM) inhibited uptake by only 25 +/- 4%. L-trans-2,4-Pyrrolidine dicarboxylate, L-serine-O-sulfate, and kainate potently inhibited transport activity with Ki values of 5.1 +/- 0.3, 56 +/- 6, and 103 +/- 9 microM, respectively (n = 3). Voltage-clamp studies of GLT1-expressing oocytes showed that, as in cortical neurons, glutamate transport was not inhibited by L-alpha-aminoadipate. Dihydrokainate was a potent inhibitor (Ki = 8 +/- 1 microM), and L-serine-O-sulfate produced a GLT1-mediated current with a K(m) value of 312 +/- 33 microM. Immunoblot analysis showed that neuronal cultures express excitatory amino acid carrier 1 (EAAC1), shown previously to be relatively insensitive to dihydrokainate, plus a trace amount of GLT1, but no GLAST. These studies establish that a major component of the glutamate transport a...Continue Reading

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Citations

Feb 14, 2003·Journal of Neuroscience Research·Toshiyuki HimiSei-Itsu Murota
Mar 11, 2004·Journal of Neurochemistry·Yaxiong YangDavid G Cook
Feb 6, 2017·Journal of Neuroscience Research·Ciaran Murphy-RoyalStéphane H R Oliet
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A C RimaniolD Dormont
Oct 18, 2002·The European Journal of Neuroscience·Yumin Zhang, Paul A Rosenberg
Nov 29, 2007·Journal of Neurochemistry·Imane NafiaLaurence Had-Aissouni
Aug 11, 2006·Alcoholism, Clinical and Experimental Research·S Saellstroem BaumClaudia D Spies
Nov 11, 2009·Current Eye Research·Shinichirou OonoOsamu Mimura
Jan 11, 2008·The European Journal of Neuroscience·Merav BassanPaul A Rosenberg
Jul 8, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R A MafraJ S Cruz
Mar 16, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Brigitte Voutsinos-PorcheAstrid Nehlig
Sep 5, 2008·The Journal of Comparative Neurology·Ann MassieYvette Michotte
Mar 1, 2014·Journal of Neural Transmission·Y Zhou, N C Danbolt
Oct 27, 2015·Frontiers in Synaptic Neuroscience·Thomas M BartolMary B Kennedy
Apr 14, 2011·The Journal of Physiology·Valérie GoubardLaurent Venance
Sep 3, 2003·Glia·Wen-Hui HuJohn R Bethea
May 23, 2001·Progress in Neurobiology·N C Danbolt
Apr 13, 2000·Neurochemistry International·J Flynn, G J McBean
Sep 16, 2009·Neurochemistry International·Karen Renata Matos OliveiraJosé Luiz Martins do Nascimento

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