High Affinity Mannotetraose as an Alternative to Dextran in ConA Based Fluorescent Affinity Glucose Assay Due to Improved FRET Efficiency

ACS Sensors
Andrea K LockeGerard L Coté

Abstract

Diabetes mellitus affects millions of people worldwide and requires that individuals tightly self-regulate their blood glucose levels to minimize the associated secondary complications. Continuous monitoring devices potentially offer patients a long-term means to tightly monitor their glucose levels. In recent years, fluorescent affinity sensors based on lectins (e.g., Concanavalin A (ConA)) have been implemented in such devices. Traditionally, these sensors pair the lectin with a multivalent ligand, like dextran, in order to develop a competitive binding assay that changes its fluorescent properties in response to the surrounding glucose concentrations. This work introduces a new type of fluorescent ligand for FRET-based assays in an attempt to improve the sensitivity of such assays. This ligand is rationally designed to present a core trimannose structure and a donor fluorophore in close proximity to one another. This design decreases the distance between the FRET donor and the FRET acceptors on ConA to maximize the FRET efficiency upon binding of the ligand to ConA. This work specifically compares the FRET efficiency and sensitivity of this new competing ligand with a traditional dextran ligand, showing that the new ligand h...Continue Reading

References

Apr 1, 1994·Analytical Biochemistry·P Wu, L Brand
Sep 20, 2000·Analytical Chemistry·R Ballerstadt, J S Schultz
Apr 28, 2005·Biosensors & Bioelectronics·John C PickupDavid J S Birch
Nov 1, 2005·Analytical Chemistry·Bennett L IbeyMichael V Pishko
Feb 21, 2006·Biosensors & Bioelectronics·Ralph BallerstadtAshok Gowda
Jun 28, 2006·Diabetes Technology & Therapeutics·Swetha Chinnayelka, Michael J McShane
Oct 3, 2007·The AAPS Journal·John W A Findlay, Robert F Dillard
Sep 10, 2008·The New England Journal of Medicine·William V TamborlaneDongyuan Xing
Feb 1, 1988·Talanta·D Meadows, J S Schultz
May 12, 2009·Diabetes Care·UNKNOWN Juvenile Diabetes Research Foundation Continuous Glucose Monitoring Study GroupDongyuan Xing
Jan 5, 2010·Journal of Diabetes Science and Technology·Jannik K NielsenKlaus Gregorius
Aug 18, 2010·International Journal of Clinical Practice. Supplement·B W Bode, T Battelino
Apr 28, 2012·Journal of Diabetes Science and Technology·Ralph Dutt-BallerstadtRoger McNichols
Feb 27, 2013·Journal of Diabetes Science and Technology·Achim Josef MüllerChristoph Hasslacher
Jul 10, 2014·Sensors·Mamdouh AloraefyKim E Sapsford
Jul 25, 2014·Biosensors & Bioelectronics·Brian M CumminsGerard L Coté
Aug 19, 2014·Analytical Chemistry·Andrea K LockeGerard L Coté

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