High-Affinity Nucleic-Acid-Based Receptors for Steroids

ACS Chemical Biology
Kyung-Ae YangMilan N Stojanovic

Abstract

Artificial receptors for hydrophobic molecules usually have moderate affinities and limited selectivities. We describe three new classes of high affinity hydrophobic receptors for nonaromatic steroids based on deoxyribonucleotides, obtained through five high stringency selections coupled with tailored counter-selections. The isolation of multiple classes of high affinity steroid receptors demonstrates the surprising breadth of moderately sized hydrophobic binding motifs (<40 nucleotides) available to natural nucleic acids. Studies of interactions with analogs indicate that two classes, four-way junctions and 4XGN motifs, comprise receptors with shapes that prevent binding of specific steroid conjugates used in counter-selections. Furthermore, they strongly prefer nonhydroxylated steroid cores, which is typical for hydrophobic receptors. The third new class accommodates hydroxyl groups in high-affinity, high-selectivity binding pockets, thus reversing the preferences of the first two classes. The high-affinity binding of aptamers to targets efficiently inhibits double-helix formation in the presence of the complementary oligonucleotides. The high affinity of some of these receptors and tailored elimination of binding through cou...Continue Reading

References

Feb 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·D W BrannV B Mahesh
May 1, 1994·Protein Science : a Publication of the Protein Society·L YoungD G Covell
Jun 11, 1999·Current Opinion in Structural Biology·M Famulok
Feb 5, 2000·Science·T Hermann, D J Patel
Apr 11, 2000·Nucleic Acids Research·T KatoI Karube
Aug 19, 2000·Nature·B YurkeJ L Neumann
Sep 9, 2000·Biochimica Et Biophysica Acta·T KatoI Karube
Feb 19, 2002·Chemical Reviews·József Szejtli
Jan 4, 2003·Nature Reviews. Molecular Cell Biology·Ralf Lösel, Martin Wehling
Jun 6, 2003·Journal of the American Chemical Society·Milan N StojanovićDonald W Landry
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Sep 8, 2004·Journal of Cellular Biochemistry·Boris J Cheskis
Apr 20, 2006·Nature Reviews. Immunology·Paul J Carter
Jul 18, 2006·Nucleic Acids Research·Oleg KikinParamjeet S Bagga
Nov 23, 2006·Journal of the American Chemical Society·Eric GreenMilan N Stojanovic
Dec 13, 2006·Science·Georg SeeligErik Winfree
Mar 17, 2007·Annals of Clinical Biochemistry·Jonathan G Middle
Jun 26, 2007·Molecular Pharmacology·Bertrand BrassartJean-François Riou
Oct 31, 2007·Methods : a Companion to Methods in Enzymology·Sattanathan ParamasivanPhilip H Bolton
Aug 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Petra VerdinoIan A Wilson
Feb 11, 2009·Nature Biotechnology·Irene NobeliJanet M Thornton
Jul 10, 2009·Chemical Communications : Chem Comm·Renjun PeiMilan N Stojanovic
Aug 24, 2010·Current Opinion in Chemical Biology·Milan N Stojanovic, Tilla S Worgall
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·David BikardDidier Mazel
Dec 7, 2011·Journal of the American Chemical Society·Kyung-Ae YangMilan N Stojanovic
Mar 28, 2012·Methods : a Companion to Methods in Enzymology·Michaela VorlíčkováJaroslav Kypr
Apr 11, 2012·International Journal of Molecular Sciences·Yu-Ming ChangMing-Hon Hou
Nov 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Douglas R DaviesThale C Jarvis
Nov 12, 2013·Biosensors & Bioelectronics·Ajeet KaushikShekhar Bhansali
Dec 4, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Katrijn VanschoenbeekLuc Michiels
May 8, 2016·Methods : a Companion to Methods in Enzymology·Kyung-Ae YangMilan N Stojanovic
May 23, 2017·Sensors·Azrul Syafiq Zainol AbidinMarimuthu Citartan
Jan 28, 2016·Biochemistry and Biophysics Reports·Mahima KaushikShrikant Kukreti

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Citations

Jul 28, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Keitel Cervantes-SalgueroMatteo Palma
Nov 10, 2020·Advanced Materials·Alexandra E RangelH Tom Soh
Aug 31, 2020·Trends in Analytical Chemistry : TRAC·Obtin AlkhamisYi Xiao
Jan 8, 2021·ACS Applied Materials & Interfaces·Nako NakatsukaAnne M Andrews
Feb 23, 2020·Analytical Biochemistry·Shima Dalirirad, Andrew J Steckl
Jun 10, 2021·Chemical Reviews·Andreas FrutigerNako Nakatsuka
Jan 7, 2021·ACS Omega·Shima DaliriradAndrew J Steckl
Jun 1, 2018·ACS Applied Materials & Interfaces·Nako NakatsukaAnne M Andrews
Mar 9, 2019·ACS Applied Materials & Interfaces·Aurora Fabry-WoodMatthew R Lakin
Oct 22, 2019·ACS Sensors·Kevin M CheungAnne M Andrews

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