PMID: 6401404Jan 1, 1983Paper

High-affinity pH-dependent passive Ca binding by myometrial plasma membrane vesicles

The American Journal of Physiology
A K GroverE E Daniel

Abstract

Rat myometrium plasma membrane-(PM) enriched fraction N1 binds calcium passively in a pH-dependent manner at a Ca2+ concentration of 1 microM. The Ca binding increases with increasing pH from 6.27 to 7.47 with a half maximum near 6.8. The difference between binding at 6.27 and 7.07 (the pH-dependent Ca binding) depends on the pH of the reaction medium rather than the pH of the medium in which the membranes had previously been suspended. The pH-dependent Ca binding is not an artifact due to EGTA, the pH buffer used, or soluble protein trapped inside the membrane vesicles. The pH-dependent Ca binding occurs with a dissociation constant value of 0.28 microM and Hill coefficient of 2.37 for Ca2+. The high affinity pH-dependent Ca uptake and the release of Ca2+ from the membranes is virtually complete in 10 s in the presence of 1 microM A23187 but not in its absence. The distribution of the pH-dependent Ca binding in the various rat myometrium subcellular fractions parallels the activity of 5'-nucleotidase in these fractions and not the activities of NADPH-dependent or succinate-dependent cytochrome c reductases. The high affinity and rapid binding and release of Ca at the pH-dependent Ca binding sites in the PM-enriched fraction su...Continue Reading

References

Oct 1, 1977·Canadian Journal of Physiology and Pharmacology·M T Goldberg, C R Triggle
Nov 18, 1976·Biochimica Et Biophysica Acta·J D Owen
Oct 1, 1980·Canadian Journal of Physiology and Pharmacology·A K GroverE E Daniel
Sep 1, 1980·Canadian Journal of Physiology and Pharmacology·A K GroverE E Daniel
Jun 1, 1980·Canadian Journal of Physiology and Pharmacology·D A CorbettD J Triggle

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Citations

Sep 1, 1996·Pflügers Archiv : European journal of physiology·M J TaggartS Wray
Dec 1, 1990·Journal of Neuroendocrinology·A LindénP Södersten
May 8, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·O Zagoory-SharonA Weller
Dec 1, 1989·Journal of Neuroendocrinology·A LindénP Sodersten
Nov 6, 2007·Molecular Biology of the Cell·Grzegorz T GurdaJohn A Williams
Jan 1, 1987·Annals of the New York Academy of Sciences·P Rosso
Dec 1, 1995·Cell Calcium·G R Monteith, B D Roufogalis
Nov 1, 1991·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·Y HabaraT Kanno

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